Oral microbiome in chinese patients with aggressive periodontitis and their family members
Oral microbiome in chinese patients with aggressive periodontitis and their family members
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DOI：
10.1111/jcpe.12463
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发表时间：
2015-11-01
影响因子：
6.7
通讯作者：
Meng, Huanxin
中科院分区：
文献类型：
--
作者：
Li, Yi;Feng, Xianghui;Meng, Huanxin
目的：调查中国侵袭性牙周炎（AgP）患者的微生物组成，并比较 AgP 患者及其家庭成员细菌谱的相似性。 材料与方法：收集 10 名患有慢性牙周炎的 AgP 患者及其 10 名一级血亲的龈下菌斑和唾液样本。生成细菌 16S rRNA 基因 V1-V3 高变区的 DNA 扩增子，并进行 454 焦磷酸测序。 结果：在龈下菌斑中，家庭成员之间的未加权 UniFrac 距离显着低于无关参与者 (p = 0.039)。与亲属相比，AgP 患者龈下菌斑和唾液的微生物群显示出明显较低的分类多样性。在 AgP 患者的龈下菌斑中检测到较高的牙龈卟啉单胞菌相对丰度（约 35.88%）。同一个体的龈下菌斑和唾液样本中牙龈卟啉单胞菌和红色复合病原体（牙龈卟啉单胞菌、齿垢密螺旋体和连翘坦纳菌）的相对丰度在AgP患者中显着相关（rho分别为0.687和0.678）。结论：中国AgP患者及其家庭成员的微生物群系统发育结构存在亲缘关系。牙龈卟啉单胞菌可能是这些中国 AgP 患者的主要病原体。
Aim: To investigate the microbiome composition in Chinese patients with aggressive periodontitis (AgP), and to compare the similarity of bacterial profiles between AgP patients and their family members.Material and Methods: Pooled subgingival plaque and saliva samples were collected from 10 AgP patients and 10 of their first-degree blood relatives with chronic periodontitis. DNA amplicons of the V1-V3 hypervariable region of the bacterial 16S rRNA gene were generated, and were subjected to 454-pyrosequencing.Results: In subgingival plaque, the unweighted UniFrac distances between family members were significantly lower than those in unrelated participants (p = 0.039). Compared with the relatives, the microbiota of subgingival plaque and saliva from AgP patients revealed significantly lower taxonomic diversity. High relative abundance of Porphyromonas gingivalis (about 35.88%) was detected in subgingival plaque from AgP patients. The relative abundance of P. gingivalis and Red complex pathogens (P. gingivalis, Treponema denticola and Tannerella forsythia) in the subgingival plaque and saliva samples from the same individual were significantly correlated in AgP patients (rho= 0.687 and 0.678, respectively).Conclusions: There is a kinship in the phylogenetic architecture of microbiota among Chinese AgP patients and their family members. P. gingivalis might be a predominant pathogen in these Chinese AgP patients.