ACTIVE OXYGEN PRODUCTION DURING A BACTERIA-INDUCED HYPERSENSITIVE REACTION IN TOBACCO SUSPENSION CELLS
ACTIVE OXYGEN PRODUCTION DURING A BACTERIA-INDUCED HYPERSENSITIVE REACTION IN TOBACCO SUSPENSION CELLS
复制标题
DOI：
10.1094/phyto-79-974
复制
发表时间：
1989-09-01
期刊：
影响因子：
3.2
通讯作者：
ATKINSON, MM
中科院分区：
文献类型：
--
作者：
KEPPLER, LD;BAKER, CJ;ATKINSON, MM
在烟草细胞悬浮液中，植物病原菌诱导的过敏反应的发生可以通过细胞外 pH 值的增加来监测。先前的报告表明，活性氧引发的脂质过氧化先于细胞外 pH 值升高。在这里，我们研究了过敏反应发生期间活性氧产生和细胞外 pH 值升高的时间。在鲁米诺存在的情况下，通过测量光产生（化学发光）来监测活性氧水平。随着时间的推移，活性氧水平的增加与细胞外 pH 值的增加相关。丁香假单胞菌 pv.丁香菌（野生型，小麦的病原体）被用来在烟草细胞悬浮液中诱导过敏反应。 P.s. syringae B7，一种不会诱导过敏反应的 Tn5 插入突变体，P. s.烟粉虱（烟草的一种病原体）被用作对照。所有细菌处理都会导致活性氧和细胞外 pH 值在 0 至 1 小时之间短暂增加。仅在经历过敏反应（接种野生型细菌）的烟草细胞悬浮液中，在 2 至 4 小时之间观察到细胞外 pH 值和活性氧水平伴随增加。
In tobacco cell suspensions, onset of the hypersensitive reaction induced by phytopathogenic bacteria can be monitored as increased extracellular pH. Previous reports suggested that active oxygen-initiated lipid peroxidation preceded this increased extracellular pH. Here, we investigated the timing of active oxygen production and increased extracellular pH during the onset of the hypersensitive reaction. Active oxygen levels were monitored by measuring light production (chemiluminescence), in the presence of luminol. Increase in active oxygen levels over time correlated with increased extracellular pH. Pseudomonas syringae pv. syringae (wild-type, a pathogen of wheat) was used to induce a hypersensitive reaction in tobacco cell suspensions. P. s. syringae B7, a Tn5 insertion mutant that does not induce a hypersensitive reaction, P. s. tabaci, a pathogen of tobacco, were used as controls. All bacterial treatments induced transient increases in both active oxygen and extracellular pH between 0 and 1 hr. Concomitant increases of extracellular pH and active oxygen levels were observed between 2 and 4 hr only in tobacco cell suspensions undergoing the hypersensitive reaction (inoculated with wild-type bacteria).