Reduced contraction of skin equivalent engineered using cell sheets cultured in 3D matrices
Reduced contraction of skin equivalent engineered using cell sheets cultured in 3D matrices
复制标题
DOI：
10.1016/j.biomaterials.2006.04.020
复制
发表时间：
2006-09-01
期刊：
影响因子：
14
通讯作者：
Hutmacher, Dietmar Werner
中科院分区：
文献类型：
--
作者：
Ng, Kee Woei;Hutmacher, Dietmar Werner
为了缓解其广泛的收缩，将人成纤维细胞片与三维基质(编织的聚乳酸-乙醇酸共聚物(PLGA)网状物和胶原-透明质酸(CHA)海绵)结合在一起，形成连续的真皮结构，用于组织工程双层皮肤等效物。所得到的构建物是可行的，并支持开发在4周培养期间不收缩的双层皮肤等效物。植入裸鼠全层创面后，基于PLGA网状物的同种异体皮肤植入率为100%，创面收缩程度与自体移植物相似，而无细胞膜的PLGA网状移植物的创面收缩程度高于自体移植物。另一方面，基于CHA海绵的皮肤替代物在移植后2周内全部脱落。在所有包含细胞片的标本中，从构建的细胞中提取的细胞在新真皮内渗透并产生细胞外基质，表现为人类白细胞抗原和I型胶原的阳性表达。这项技术为基于支架的组织工程提供了一种替代方法，可以用成熟的新组织制作机械稳定的移植物。(C)2006爱思唯尔有限公司。保留所有权利。
In order to alleviate their extensive contraction, human fibroblast sheets were cultured in combination with three-dimensional matrices (knitted poly(lactic-co-glycolic acid) (PLGA) mesh and collagen-hyaluronic acid (CHA) sponge) to form contiguous dermal constructs for tissue engineering a bilayered skin equivalent. The resulting constructs were viable, and supported the development of bilayered skin equivalents which did not contract over the 4-week culture period. When implanted into full-thickness wounds in nude rats, cultured skin equivalents based on PLGA meshes registered a take rate of 100% and showed an extent of wound contraction that was statistically similar to autografts, while wounds grafted with PLGA meshes without cell sheets contracted more than autografts. On the other hand, skin equivalents based on CHA sponges were all sloughed off within 2 weeks of transplantation. In all cell sheet-incorporated specimens, cells from the constructs infiltrated and produced extracellular matrix within the neo-dermis, shown by positive human leukocyte antigen and collagen I expression. This technique offers an alternative approach for scaffold-based tissue engineering to produce mechanically stable grafts with matured neo-tissue. (c) 2006 Elsevier Ltd. All rights reserved.