Replication-dependent instability at (CTG) x (CAG) repeat hairpins in human cells.
Replication-dependent instability at (CTG) x (CAG) repeat hairpins in human cells.
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DOI：
10.1038/nchembio.416
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发表时间：
2010-09
影响因子：
14.8
通讯作者：
Leffak, Michael
中科院分区：
文献类型：
--
作者：
Liu, Guoqi;Chen, Xiaomi;Bissler, John J.;Sinden, Richard R.;Leffak, Michael
Instability of (CTG)•(CAG) microsatellite trinucleotide repeat (TNR) sequences is responsible for more than one dozen neurological or neuromuscular diseases. TNR instability during DNA synthesis is thought to involve slipped strand or hairpin structures in template or nascent DNA strands, although direct evidence for hairpin formation in human cells is lacking. We have used targeted recombination to create a series of isogenic HeLa cell lines in which (CTG)•(CAG) repeats are replicated from an ectopic copy of the c-myc replication origin. In this system the tendency of chromosomal (CTG)•(CAG) tracts to expand or contract was affected by origin location and the leading or lagging strand replication orientation of the repeats, and instability was enhanced by prolonged cell culture, increasing TNR length, and replication inhibition. Hairpin cleavage by synthetic zinc finger nucleases in these cells has provided the first direct evidence for the formation of hairpin structures during replication in vivo.
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影响因子：
3.5
作者：
Libby, RT;Monckton, DG;La Spada, AR
通讯作者：
La Spada, AR
影响因子：
9.8
作者：
Bidichandani, SI;Ashizawa, T;Patel, PI
通讯作者：
Patel, PI
DOI：
10.1073/pnas.95.21.12438
发表时间：
1998-10-13
影响因子：
11.1
作者：
Miret, JJ;Pessoa-Brandao, L;Lahue, RS
通讯作者：
Lahue, RS
影响因子：
3.5
作者：
Khajavi, M;Tari, AM;Ashizawa, T
通讯作者：
Ashizawa, T
DOI：
10.1080/10937400601171241
发表时间：
2007-01-01
影响因子：
2.6
作者：
Carbajal Rivera, Maria Luisa;Ordaz Tellez, Maria Guadalupe;Rodriguez-Arnaiz, Rosario
通讯作者：
Rodriguez-Arnaiz, Rosario