Dibutyl phthalate degradation by Enterobacter sp T5 isolated from municipal solid waste in landfill bioreactor
Dibutyl phthalate degradation by Enterobacter sp T5 isolated from municipal solid waste in landfill bioreactor
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DOI：
10.1016/j.ibiod.2010.04.010
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Shen, Dong-Sheng
中科院分区：
文献类型：
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作者：
Fang, Cheng-Ran;Yao, Jun;Shen, Dong-Sheng
从垃圾填埋场生物反应器中分离到一株能利用邻苯二甲酸二丁酯（DBP）作为唯一碳源和能源的细菌。根据其形态特征、生理生化特征和165 rDNA序列分析，将该菌株鉴定为肠杆菌属（Enterobacter sp.）T5。研究了几种环境因素对DBP微生物降解的影响。其生物降解活性的最适pH和温度分别为7.0和35 ℃。初始DBP浓度对降解的影响进行了研究之间的100和1500毫克l（-1）。结果表明，DBP的降解符合一级动力学模型，当DBP浓度低于1000 mg l（-1）时，降解半衰期为20.9 h。经初步鉴定，肠杆菌T5降解DBP的主要产物为PA和MBP。肠杆菌T5也能以邻苯二甲酸二甲酯、邻苯二甲酸二乙酯或邻苯二甲酸二辛酯溶液作为唯一碳源和能量源生长，这表明其具有抵抗邻苯二甲酸二酯（PAE）毒性的能力。这些结果表明，肠杆菌属T5可以作为候选人修复含PAE的废物。(C)2010爱思唯尔有限公司版权所有。
A bacterial strain capable of utilizing dibutyl phthalate (DBP) as its sole source of carbon and energy was isolated from municipal solid waste in a landfill bioreactor. Based on its morphology, physiochemical characteristics, and 165 rDNA sequence, the strain was identified as Enterobacter sp. T5. Microbial degradation of DBP was studied for several environmental factors. The optimal pH and temperature for its biodegradation activities were 7.0 and 35 degrees C, respectively. The effect of initial DBP concentrations on the degradation was investigated between 100 and 1500 mg l(-1). The results showed that the degradation fit a first-order kinetic model, and the degradation half-life was about 20.9 h when the concentration of DBP was lower than 1000 mg l(-1). The major DBP degradation products by Enterobacter sp. T5 were preliminarily identified as PA and MBP. Enterobacter sp. T5 also grew with dimethyl phthalate, diethyl phthalate or dioctyl phthalate solution as the sole source of carbon and energy, which suggested its ability to resist phthalic acid diester (PAE) toxicities. These results suggested that Enterobacter sp. T5 could be used as a candidate for remediating PAE-containing wastes. (C) 2010 Elsevier Ltd. All rights reserved.