Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses
Vibrio cholerae hemagglutinin/protease degrades chironomid egg masses
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DOI：
10.1128/aem.69.7.4200-4204.2003
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Kashi, Y
中科院分区：
文献类型：
--
作者：
Halpern, M;Gancz, H;Kashi, Y
霍乱是一种严重的肠道疾病，由对人类具有致病性的特定血清群的霍乱弧菌引起。这种疾病不会在人类或动物中持续存在。病原体在自然界中是一种自由生活的有机体。最近，有人建议，卵块的nonbiting摇蚊属（双翅目）港口，并作为V cholesterol的营养来源，从而提供了一个自然水库的有机体。在这里，我们报告V cholesterol O 9，O 1，和O 139上清液裂解的凝胶状基质的摇蚊卵团，并抑制孵化卵。从霍乱弧菌O 9和O 139中分离纯化了降解摇蚊卵团的胞外因子（卵团降解因子），并鉴定为霍乱弧菌主要分泌的血凝素/蛋白酶（HA/P）。卵块中的底物被表征为糖蛋白。这些结果表明，RA/P在V胆碱和摇蚊卵团的相互作用中起着重要作用。
Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the nonbiting midge Chironomus sp. (Diptera) harbor and serve as a nutritive source for V cholerae, thereby providing a natural reservoir for the organism. Here we report that V cholerae O9, O1, and O139 supernatants lysed the gelatinous matrix of the chironomid egg mass and inhibited eggs from hatching. The extracellular factor responsible for the degradation of chironomid egg masses (egg mass degrading factor) was purified from V cholerae O9 and O139 and was identified as the major secreted hemagglutinin/protease (HA/P) of V cholerae. The substrate in the egg mass was characterized as a glycoprotein. These findings show that RA/P plays an important role in the interaction of V cholerae and chironomid egg masses.