Precise microinjection into skin using hollow microneedles
Precise microinjection into skin using hollow microneedles
复制标题
DOI：
10.1038/sj.jid.5700150
复制
发表时间：
2006-05-01
影响因子：
6.5
通讯作者：
Prausnitz, Mark R.
中科院分区：
文献类型：
--
作者：
Wang, Ping M.;Cornwell, Megan;Prausnitz, Mark R.
微米尺寸的中空针先前已经被制造并设想用于与透皮贴剂或输液泵一起使用，以实现药物到皮肤的无痛递送，用于局部和全身作用，而不需要皮下注射针。然而，很少有工作已经进行，以确定有效地使用空心微针的药物输送的方法。为了满足这一需求，我们将中空的玻璃微针插入无毛大鼠体内皮肤和人尸体体外皮肤，然后通过明场和荧光显微镜成像染料分子，胰岛素，聚合物微粒和细胞注入皮肤。通过用旋转钻孔装置插入针来控制针穿透到皮肤中的深度，该旋转钻孔装置使得能够以760 μ m/m的分辨率在表皮或真皮内进行局部注射。尽管在针插入皮肤后可以注射少量的流体，但是通过回缩100-300 μ m或振动微针阵列来部分回缩针显著地增加了输注流速。我们的结论是，空心微针可用于精确的微注射到皮肤中，特别是当一个单一的针插入旋转钻孔，然后回缩前输液或微针阵列插入机械振动的部分方式。
Hollow needles of micron dimensions have previously been fabricated and envisioned for use with transdermal patches or infusion pumps to achieve painless delivery of drugs to the skin for local and systemic effects without the need for hypodermic needles. However, little work has been carried out to identify methods to effectively use hollow microneedles for drug delivery. To address this need, we inserted hollow, glass microneedles into hairless rat skin in vivo and human cadaver skin in vitro and then imaged infusion of dye molecules, insulin, polymer microparticles, and cells into the skin by brightfield and fluorescence microscopy. The depth of needle penetration into skin was controlled by inserting needles with a rotary drilling device, which enabled localized injection within the epidermis or dermis with 760 m m resolution. Although small quantities of fluid could be injected after needle insertion into skin, partial retraction of the needle by withdrawing back 100-300 mu m or vibrating the microneedle array dramatically increased infusion flow rate. We conclude that hollow microneedles can be used for precise microinjection into skin, especially when a single needle is inserted by rotary drilling and then retracted part way before infusion or a microneedle array is inserted by mechanical vibration.