Two-phase endolymphatic hydrops: A new dynamic guinea pig model
Two-phase endolymphatic hydrops: A new dynamic guinea pig model
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DOI：
10.3109/00016489709117984
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发表时间：
1997-01-01
影响因子：
1.4
通讯作者：
Albers, FWJ
中科院分区：
文献类型：
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作者：
Dunnebier, EA;Segenhout, JM;Albers, FWJ
经典的梅尼埃氏病豚鼠模型是通过破坏内淋巴囊和堵塞内淋巴管来实现内淋巴水肿的，这是一种非生理性的深度模型，与患者的梅尼埃氏病有关。我们开发了一个更精细的动物模型；两相内淋巴积液。该模型是基于慢性内淋巴囊功能障碍（由内淋巴囊最末端的轻微破坏引起）和急性应激诱导的内淋巴生成（由醛固酮刺激血管纹中的Na/ k - atp酶引起）的组合。用光镜检查四组耳蜗液室是否存在内淋巴积液：i)正常（对照）耳蜗未见积液；Ii)部分未手术（未破坏）醛固酮处理耳蜗，主要在基底匝部出现小程度积水；Iii)在不使用醛固酮的情况下，内淋巴囊轻度剥离产生积水，主要存在于耳蜗尖；慢性内淋巴囊功能障碍与醛固酮引起的急性内淋巴生成发作相结合，显示出最严重程度的耳蜗袢水肿、耳蜗结构损伤和内淋巴囊上皮内层细胞紊乱。这个新模型可能代表了一种更生理和动态的方法来研究梅尼埃氏病，并可能解释患者的许多症状的病因，如波动的性质和感觉神经性听力损失的类型。
The classical guinea pig model for Meniere's disease, in which endolymphatic hydrops was achieved by destruction of the endolymphatic sac and obliteration of the endolymphatic duct, is a non-physiological profound model with shortcomings in relation to Meniere's disease as seen in patients. We developed a more subtle animal model; the two-phase endolymphatic hydrops. This model is based on a combination of chronic endolymphatic sac dysfunction, induced by slight destruction of the most distal part of the endolymphatic sac, and acute stress-induced endolymph production by stimulation of the Na/K-ATPase in the stria vascularis with aldosterone. Light microscopy of the fluid compartments of four groups of cochleas was used to examine them for the presence of endolymphatic hydrops: i) Normal (control) cochleas showed no hydrops; ii) some of the non-operated (no destruction) aldosterone-treated cochleas showed small degrees of hydrops mainly present in the basal turns; iii) mild dissection of the endolymphatic sac without administration of aldosterone produced a hydrops which was mainly present in the cochlear apex; iv) combination of chronic endolymphatic sac dysfunction and acute attacks of endolymph production by aldosterone administration revealed the most severe degrees of hydrops in ail cochlear windings, damage to cochlear structures, and cellular disturbances of the epithelial lining of the endolymphatic sac. This new model may represent a more physiologic and dynamic approach to Meniere's disease and may explain the etiology of many symptoms in patients such as the fluctuant nature and the types of sensoneurinal hearing losses.