In vivo MRI volumetric measurement of prostate regression and growth in mice.
In vivo MRI volumetric measurement of prostate regression and growth in mice.
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DOI：
10.1186/1471-2490-7-12
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发表时间：
2007-07-24
期刊：
影响因子：
2
通讯作者：
Krolewski JJ
中科院分区：
文献类型：
--
作者：
Nastiuk KL;Liu H;Hamamura M;Muftuler LT;Nalcioglu O;Krolewski JJ
背景用于治疗晚期前列腺癌的小鼠模型是有价值的工具，但是由于前列腺原位的位置、小尺寸和交叉解剖结构，评估前列腺的生长程度，特别是由于治疗干预或去势导致的其消退是困难的。小鼠前列腺退化的时间监测需要多个动物和组织切片的检查。首先，对正常年龄的C57/BL 6小鼠进行T2加权磁共振成像（MRI）。使用吸入麻醉重复对个体小鼠进行成像，以建立方法的再现性并遵循前列腺体积的激素操作。随后，使用化学位移选择性（CHESS）脉冲抑制MRI脂肪信号，以避免信号污染并增强前列腺的辨别力。高场（7 T）MRI可提供正常小鼠前列腺的高分辨率（平面内117 × 117 μm）、高重现性图像。尽管成像时间长，但动物可以重复成像以建立体积测量的可靠性。前列腺体积在去势后下降，随后在同一动物中给予雄激素后恢复正常。CHESS成像允许区分前列腺边缘和前列腺背侧叶（DLP）与腹侧叶（VP）。去势导致DLP体积减少40%，VP体积减少75%。小鼠前列腺体积的MRI评估是精确和可重复的。MRI改善了消退程度的体积测定，并且可以在治疗过程中随时间监测同一小鼠。由于避免了在每个时间点评估动物组，这提高了任何操作效果的测量的准确性，并减少了所需的动物数量。
Background Mouse models for treatment of late-stage prostate cancer are valuable tools, but assessing the extent of growth of the prostate and particularly its regression due to therapeutic intervention or castration is difficult due to the location, small size and interdigitated anatomy of the prostate gland in situ. Temporal monitoring of mouse prostate regression requires multiple animals and examination of histological sections. Initially, T2-weighted magnetic resonance imaging (MRI) was performed on normal year-old C57/BL6 mice. Individual mice were repeatedly imaged using inhalation anesthesia to establish the reproducibility of the method and to follow hormone manipulation of the prostate volume. Subsequently, MRI fat signal was suppressed using a chemical shift-selective (CHESS) pulse to avoid signal contamination and enhance discrimination of the prostate. High field (7T) MRI provides high resolution (117 × 117 μm in plane), highly reproducible images of the normal mouse prostate. Despite long imaging times, animals can be imaged repeatedly to establish reliability of volume measurements. Prostate volume declines following castration and subsequently returns to normal with androgen administration in the same animal. CHESS imaging allowed discrimination of both the margins of the prostate and the dorsal-lateral lobes of the prostate (DLP) from the ventral lobes (VP). Castration results in a 40% reduction in the volume of the DLP and a 75% reduction in the volume of the VP. MRI assessment of the volume of the mouse prostate is precise and reproducible. MRI improves volumetric determination of the extent of regression and monitoring of the same mouse over time during the course of treatment is possible. Since assessing groups of animals at each time point is avoided, this improves the accuracy of the measurement of any manipulation effect and reduces the number of animals required.