Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture
Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture
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DOI：
10.1038/nprot.2007.256
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Paguirigan, Amy L.;Beebe, David J.
我们开发了一种利用天然交联法从明胶制造微流控器件的技术。通过使用标准光刻技术生产可重复使用的聚二甲基硅氧烷模具，明胶可以模制成微通道几何形状。这种明胶通过一种简单的方法与自然产生的谷氨酰胺转氨酶进行交联，从而产生适合细胞培养的设备。该方案从明胶制备开始到细胞种植大约需要1天。使用这些设备，可以在更接近体内环境的微环境中研究细胞外基质和可溶性因子对细胞行为和分化的影响。
We have developed a technique for fabricating microfluidic devices from gelatin using a natural crosslinking process. By producing reusable poly(dimethyl siloxane) molds using standard photolithography, gelatin can be molded into microchannel geometries. The gelatin is crosslinked with the naturally occurring enzyme transglutaminase via a straightforward process that can produce devices suitable for cell culture. The protocol takes approximately 1 day from the start of gelatin preparation to cell seeding. Using these devices, the effects of both the extracellular matrix and soluble factors on cellular behavior and differentiation can be studied in microenvironments that more closely mimic the in vivo environment.