Biological and phylogenetic characterization of a novel hemagglutination-negative avian avulavirus 6 isolated from wild waterfowl in China
Biological and phylogenetic characterization of a novel hemagglutination-negative avian avulavirus 6 isolated from wild waterfowl in China
复制标题
从中国野生水禽中分离出的新型血凝阴性禽鸟病毒 6 的生物学和系统发育特征。
DOI：
10.1111/tbed.13005
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发表时间：
2018-12-01
影响因子：
4.3
通讯作者：
Yin, Renfu
中科院分区：
文献类型：
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作者：
Chen, Yanyu;Ding, Zhuang;Yin, Renfu
自1977年在中国香港地区的家鸭身上首次发现禽6型病毒（AAvV-6/duck/HongHong Kong/18/199/77）以来，迄今为止，世界上只发现了9个AAvV-6的全基因组序列。由于AAvV-6的分离率较低，对养禽业造成的损失较小，因此对AAvV-6的传播途径、遗传学和生物学特性的了解非常有限。为更好地探讨上述因素之间的关系，2013-2017年对中国6个疑似种间传播点和洲际飞行通道的家禽和野鸟进行了AAvV-6流行病学监测。因此，分别采集野生鸟类粪便样本9，872份和活禽市场临床健康家禽泄殖腔和气管拭子样本1，642份。然而，仅从湖北省湿地野鸭的新鲜粪便样品中分离到一种新的血凝阴性AAvV-6分离株（AAvV-6/mallard/Hubei/2015）。对该AAvV-6分离株（AAvV-6/mallard/Hubei/2015）的序列测定和系统发育分析表明，该分离株属于基因I型，与欧美野鸟源病毒存在洲际流行病学连锁。同时，在血清型AAvV-6中存在至少两种基因型（I和II）。此外，当用胰蛋白酶预处理时，鸡胚中的这种新的血凝阴性AAvV-6分离株恢复了其血凝。这些研究结果，以及来自其他AAvV-6的数据，表明野生候鸟传播AAvV-6的潜在流行病学洲际传播，并揭示了对全球野生鸟类和家禽的潜在威胁。
Up to now only nine whole genome sequences of avian avulavirus 6 (AAvV-6) had been documented in the world since the first discovery of AAvV-6 (AAvV-6/duck/HongKong/18/199/77) at a domestic duck in 1977 from Hong Kong of China. Very limited information is known about the regularities of transmission, genetic and biological characteristics of AAvV-6 because of the lower isolation rate and mild losses for poultry industry. To better further explore the relationships among above factors, an AAvV-6 epidemiological surveillance of domestic poultry and wild birds in six provinces of China suspected of sites of inter-species transmission and being intercontinental flyways during the year 2013-2017 was conducted. Therefore, 9,872 faecal samples from wild birds and 1,642 cloacal and tracheal swab samples from clinically healthy poultry of live bird market (LBM) were collected respectively. However, only one novel hemagglutination-negative AAvV-6 isolate (AAvV-6/mallard/Hubei/2015) was isolated from a fresh faecal sample obtained from mallard at a wetland of Hubei province. Sequencing and phylogenetic analyses of this AAvV-6 isolate (AAvV-6/mallard/Hubei/2015) indicated that this isolate grouping to genotype I were epidemiological intercontinentally linked with viruses from the wild birds in Europe and America. Meanwhile, at least two genotypes (I and II) are existed within serotype AAvV-6. In additional, this novel hemagglutination-negative AAvV-6 isolate in chicken embryos restored its hemagglutination when pre-treated with trypsin. These findings, together with data from other AAvV-6, suggest potential epidemiological intercontinental spreads among AAvV-6 transmission by wild migratory birds, and reveal potential threats to wild birds and domestic poultry worldwide.