Dissection of the neuron network in the catfish inner retina. IV. Bidirectional interactions between amacrine and ganglion cells.
Dissection of the neuron network in the catfish inner retina. IV. Bidirectional interactions between amacrine and ganglion cells.
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鲶鱼视网膜内神经元网络的解剖。
DOI：
10.1152/jn.1990.63.1.105
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发表时间：
1990
影响因子：
2.5
通讯作者：
Naka,KI
中科院分区：
文献类型：
--
作者：
Sakai,HM;Naka,KI
1. 采用电流注射的方法对鲶鱼视网膜内的神经元网络进行了功能解剖。同时用两个单独的电极对两个相邻的神经元进行细胞内记录。将外部电流注入一个神经元，并从另一个神经元记录细胞内反应。测试信号包括1)单频正弦波，2)去极化或超极化电流脉冲，以及3)白噪声调制电流，通过输入-输出互相关过程计算维纳核。2. 外源性电流注入上神经节（NA）细胞引起邻近上神经节（GA）细胞的反应。相反，注入GA细胞的电流会引起邻近NA细胞的反应。在OFF无腺细胞（NB）和OFF神经节细胞（GB）之间的传递也得到了类似的结果。无突细胞和神经节细胞之间具有相同响应极性的正向和反向传输的神经滤波器为低通、恒增益，截止频率为40-50 Hz。通过电流振幅关系测量的增益可与正向（N----G）和反向（G----N）传输相媲美。3. 在无突细胞之间和具有相同反应极性的神经节细胞之间发现了类似的双向信号传递。用于这种传输的神经滤波器也是低通，恒定增益，截止频率为40-50 Hz。由于大部分电流诱发的反应是由一阶核预测的，因此相同反应极性的细胞之间的传递近似线性。与光诱发的一阶核相比，电流诱发的一阶核更短，更像脉冲。5. 我们的结论是，腺分泌和神经节细胞的开和关形成两个开和关细胞簇，其中细胞广泛且双向连接，增强了每个簇的反应。
1. We have functionally dissected the neuron network in the catfish inner retina by means of current injection. Simultaneous intracellular recordings were made from two neighboring neurons with the use of two separate electrodes. Extrinsic current was injected into one neuron, and the resulting intracellular responses were recorded from the other neuron. The test signals included 1) a single-frequency sinusoid, 2) a depolarizing or a hyperpolarizing current pulse, and 3) white-noise modulated current from which Wiener kernels were computed by an input-output cross-correlation process. 2. Extrinsic current injected into an ON amacrine (NA) cell evoked responses from a neighboring ON ganglion (GA) cell. Conversely, current injected into a GA cell elicited responses from a neighboring NA cell. Similar results were obtained for the transmission between OFF amacrine (NB) and OFF ganglion (GB) cells. Neural filters for the forward and backward transmissions between amacrine and ganglion cells of the same response polarity were low-pass, constant gain with a cutoff frequency of 40-50 Hz. The gain measured by current-amplitude relationships was comparable for the forward (N----G) and backward (G----N) transmission. 3. Similar bidirectional signal transmission was found between amacrine cells and between ganglion cells of the same response polarity. Neural filters for such transmission were also low-pass, constant gain with a cutoff frequency of 40-50 Hz. 4. Because a large portion of the current-evoked response was predicted by the first-order kernel, transmission between cells of the same response polarity was approximately linear. The current-evoked first-order kernels were brief and impulse-like compared with the light-evoked first-order kernels. 5. We conclude that ON and OFF amacrine and ganglion cells form two ON- and OFF-cell clusters in which cells are extensively and bidirectionally interconnected, enhancing the response in each cluster.