Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date Palm Pollen (DPP) from two Tunisian cultivars
Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date Palm Pollen (DPP) from two Tunisian cultivars
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DOI：
10.1016/j.arabjc.2015.07.014
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发表时间：
2019-12-01
影响因子：
6
通讯作者：
Gharsallah, Neji
中科院分区：
文献类型：
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作者：
Daoud, Amal;Malika, Drira;Gharsallah, Neji
本研究旨在研究和比较从突尼斯品种 Kerkennah (DPP-K) 和 Tozeur (DPP-T) 中使用各种溶剂（包括己烷、氯仿、乙酸乙酯、丙酮、乙醇和水）采集的海枣花粉中回收的不同提取物的总酚含量（TPC）和总黄酮含量（TFC）以及抗氧化和抗菌活性。结果显示，对于除己烷之外的所有溶剂提取物，DPP-T 的 TPC 均高于 DPP-K，水提取物的 TPC 值为 237.74 +/- 9.58 mg GAE/g。丙酮 DPP-T 提取物中的 TFC 含量最高（75.10 +/- 4.37 mg QE/g），大约是 DPP-K 的两倍。通过 HPLC 鉴定和定量了两种提取物中总共八种酚类化合物，即没食子酸、儿茶素、咖啡酸、表儿茶素、香草酸、香豆素、槲皮素和芦丁。使用 DPPH 和 β-胡萝卜素漂白测定法筛选提取物的抗氧化活性。结果表明，DPP-T 丙酮提取物显示出最佳的 DPPH 清除活性（IC50 = 46.56 +/- 0.28 mu g/ml），而 DPP-K 没有记录到活性。与作为参考标准的 BHT 相比，DPP-T 提取物在 β-胡萝卜素测试 (28.12 +/- 0.04 mu g/ml) 方面也显示出显着的效果。筛选提取物对10种细菌和7种真菌菌株的抗菌活性，结果显示，乙酸乙酯DPP-K提取物对单增李斯特菌的活性最强，金黄色葡萄球菌菌株对DPP-T最敏感，MIC和MBC值分别为0.98 mg/ml和1.95 mg/ml。 DPP-T和DPP-K对尖孢菌的生长均表现出较强的抑制作用。进一步的时间杀灭测定证明了 DPP-K 和 DPP-T 乙酸乙酯提取物分别抑制单核细胞增生李斯特菌和金黄色葡萄球菌生长的效力。总体而言，研究结果表明，DPP 可被视为新型天然抗氧化剂和抗菌剂的有前景的来源，可用于各种食品和药品及配方。这表明 DPP 提取物是食品腐败微生物生长的良好潜在抑制剂，并且可能成为人类和植物感染的高效治疗选择。 (C) 2015 年作者。由 Elsevier B.V. 出版
This study aimed to investigate and compare the total phenolic content (TPC) and total flavonoid content (TFC) and antioxidant and antibacterial activities of different extracts recovered from Date Palm Pollen collected from Tunisian cultivars of Kerkennah (DPP-K) and Tozeur (DPP-T) using various solvents, including hexane, chloroform, ethyl acetate, acetone, ethanol and water. The results revealed that DPP-T had higher TPC than DPP-K for all solvent extracts, except for hexane, with a value of 237.74 +/- 9.58 mg GAE/g for the water extract. The highest level of TFC (75.10 +/- 4.37 mg QE/g) was recorded in the acetone DPP-T extract, which was about twice as high as that of DPP-K. A total of eight phenolic compounds were identified and quantified in both extracts by HPLC, namely gallic acid, catechin, caffeic acid, epicatechin, vanillic acid, coumarin, quercitin and rutin. The antioxidant activities of the extracts were screened using DPPH and beta-carotene bleaching assays. The results indicated that the DPP-T acetone extract showed the best DPPH scavenging activity (IC50 = 46.56 +/- 0.28 mu g/ml), with no activity being recorded for DPP-K. The DPP-T extract also showed significant effects in terms of the beta-carotene test (28.12 +/- 0.04 mu g/ml) when compared to BHT as a reference standard. The extracts were screened for antimicrobial activity against 10 bacterial and 7 fungal strains, and the results showed that ethyl acetate DPP-K extract exhibited the strongest activity against Listeria monocytogenes and that the Staphylococcus aureus strain was most sensitive to DPP-T, with MIC and MBC values of 0.98 mg/ml and 1.95 mg/ml, respectively. Both DPP-T and DPP-K showed strong inhibition effects on the growth of oxysporum. Further time kill assays demonstrated the potency of DPP-K and DPP-T ethyl acetate extracts to inhibit the growth of L. monocytogenes and S. aureus, respectively. Overall, the findings suggest that DPP could be considered a promising source of new natural antioxidant and antimicrobial agents for use in various food and pharmaceutical products and formulations. This suggested that the DPP extract is a good potential inhibitor of food spoiling microbial growth and could be a highly effective therapeutic choice for human and plant infections. (C) 2015 The Authors. Published by Elsevier B.V.