Changed Growth Characteristics with Bacillus thuringiensis Gene Introduction and Nitrogen Regulation in Bt Cotton
Changed Growth Characteristics with Bacillus thuringiensis Gene Introduction and Nitrogen Regulation in Bt Cotton
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苏云金芽孢杆菌基因导入和氮素调控改变 Bt 棉花的生长特性
DOI：
10.2134/agronj2016.04.0209
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发表时间：
2017-01-01
期刊：
影响因子：
2.1
通讯作者：
Chen, De-Hua
中科院分区：
文献类型：
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作者：
Abidallha, Eltayib;Tambel, Leila I. M.;Chen, De-Hua
苏云金芽孢杆菌(Bt)转基因棉花(Gossypium hirsutom L.)的营养生长和生殖生长都发生了变化。本研究的目的是确定转Bt基因棉花后的营养生长和生殖生长特性，并研究氮素对营养生长和生殖生长的影响，以确定氮素营养是否为转Bt基因棉花生长特性的改变提供了潜在的机制。2011~2012年间，选取3个Bt品种(思抗1号、Ccsi41和Ccsi45)及其当前亲本(思棉3号、Ccsi23和吉棉7号)进行了营养生长和生殖生长特性及氮素分配的比较研究，并在此基础上设计了两个施氮量(0和450 kg/ha)处理，以研究2013年氮素调控对生长的影响。结果表明，转Bt基因抗虫棉品种的主茎叶面积、合生叶数和株高的生长速率均高于其亲本，而合生叶面积的生长速率低于其亲本。结果节数、果枝数、结铃率和残铃率均高于亲本，但果枝位置低于亲本。缺氮和富氮处理对Bt品种的营养生长和生殖生长均有影响。与亲本相比，Bt品种在缺氮条件下营养器官和生殖器官的数量和比例都有所降低。这些结果表明，Bt基因的导入改变了植株的生长特性，包括在缺氮条件下营养器官和生殖器官的发育减少。
Changes in vegetative and reproductive growth have been widely observed in Bacillus thuringiensis (Bt) transgenic cotton (Gossypium hirsutum L.). The objectives of this study were to determine the characteristics of vegetative and reproductive growth aft er the Bt gene was introduced into cotton and to study the effect of N on vegetative and reproductive growth to determine if N nourishment could provide a potential mechanism for changed growth characteristics in Bt cotton. Three Bt cultivars (Sikang1, Ccsi41, and Ccsi45), with their current parents (Simian3, Ccsi23, and Jimian7, respectively), were chosen to compare the characteristics of vegetative and reproductive growth and N distribution, in 2011 to 2012; the same six cultivars with two N fertilizer application rate treatments (0 and 450 kg/ha) were further designed to investigate the N regulation impact on growth in 2013. The results indicated that the Bt cotton cultivars had higher growth rates of main stem leaf area, sympodium leaf number, and plant height and a lower growth rate of sympodium leaf area than their parents. Higher numbers and development rates of fruiting nodes, fruiting branches, bolls, and retained boll rates were also observed but lower fruiting branch positions than their parents. The N-deficit and N-rich treatments affected both vegetative and reproductive growth of Bt cultivars. The Bt cultivars had reduced numbers and rates of vegetative and reproductive organs under N deficit compared with their parents. These results show that the introduction of the Bt gene changed growth characteristics, including reduced vegetative and reproductive organ development under N deficit.