Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall
Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall
复制标题
灵芝和酿酒酵母发酵对油菜花粉形态及其壁的影响
DOI：
10.1007/s13197-017-2868-1
复制
发表时间：
2017-09
影响因子：
3.1
通讯作者：
Zhao Fengyun
中科院分区：
文献类型：
--
作者：
Zhang Zheng;Cao Honggang;Chen Chao;Chen Xiao;Wei Qi;Zhao Fengyun
This study investigated the mechanism of wall rupture fermentation of the rape bee pollen by Ganoderma lucidum and Saccharomyces cerevisiae. The enzymatic activities and broken-wall ratios were determined, and the results suggested the involvement of cellulase, protease, and pectinase in the wall rupture fermentation. Among the five substrate inducers, CMCase, protease, and pectinase had the highest enzymatic activities at 23.13 ± 1.09, 63.44 ± 1.35, and 118.61 ± 2.07 U/mL, respectively. The broken-wall ratios of G. lucidum and S. cerevisiae were 85.08 ± 3.92 and 88.31 ± 2.51%, respectively. The wall rupture of rape pollen was closely related to the enzymes of the fermenting species. The wall rupture process was determined through enzyme assay, light microscopy, and scanning electron microscopy, and the broken-wall ratios provide further information on the said mechanism. The process follows three steps, namely, removal of coating on the pollen surface, continuous opening of three germinal apertures to allow enzymatic breakdown of the intine, and release of contents from the degradation of the exine.
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DOI：
10.6165/tai.1999.44(1).1
发表时间：
1999-03
期刊：
--
影响因子：
--
作者：
J. Rowley;D. Claugher;J. Skvarla
通讯作者：
J. Rowley;D. Claugher;J. Skvarla
影响因子：
6.6
作者：
Xiangying Xu;Liping Sun;Jie Dong;Hongcheng Zhang
通讯作者：
Xiangying Xu;Liping Sun;Jie Dong;Hongcheng Zhang
影响因子：
--
作者：
H. Brockman
通讯作者：
H. Brockman
影响因子：
6.1
作者：
H. Nagata;T. Takei;Ryoko Kojima;Y. Kodera;H. Nishimura;Y. Inada;A. Matsushima
通讯作者：
H. Nagata;T. Takei;Ryoko Kojima;Y. Kodera;H. Nishimura;Y. Inada;A. Matsushima
影响因子：
0.9
作者：
E. Duhoux
通讯作者：
E. Duhoux