A gridded genomic library of the honeybee (Apis mellifera):: A reference library system for basic and comparative genetic studies of a hymenopteran genome
A gridded genomic library of the honeybee (Apis mellifera):: A reference library system for basic and comparative genetic studies of a hymenopteran genome
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DOI：
10.1006/geno.1998.5253
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发表时间：
1998-04-15
期刊：
影响因子：
4.4
通讯作者：
Lehrach, H
中科院分区：
文献类型：
--
作者：
Beye, M;Poch, A;Lehrach, H
我们提出了一个网格化的蜜蜂（Apis mellifera）基因组文库，用于蜜蜂基因组的比较和基础遗传学研究。该图书馆将作为一个“参考图书馆”系统建立，克隆和数据将与整个科学界共享。这将加速蜜蜂遗传学的分子水平，结合不同实验室的努力。由于雄性单倍性和高重组率，蜜蜂正成为自然发生性状和行为遗传学基因组研究的模式生物。该文库由约110，000个克隆组成，这些克隆以高密度点样到四个滤膜上，代表22个基因组当量。使用单拷贝序列的初步分析显示了相同数量级的阳性克隆数。库生成和初步分析以及库访问的技术进行了描述。(C)北京：科学出版社.
We present a gridded genomic library of the honeybee (Apis mellifera) for comparative and basic genetic study of the honeybee genome. The library will be established as a "Reference Library" system, and clones as well as data will be shared with the entire scientific community. This will accelerate the molecular level of honeybee genetics, combining the efforts of different laboratories. Because of male haploidy and the high rate of recombination, the honeybee is becoming a model organism for genomic studies of naturally occurring traits and behavioral genetics. The library consists of about 110,000 clones spotted at high density onto four filter membranes, representing 22 genome equivalents. Preliminary analysis using single-copy sequences revealed a positive clone number of the same order. The techniques for library generation and preliminary analysis as well as library access are described. (C) 1998 Academic Press.