Multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen Candida guilliermondii
Multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen Candida guilliermondii
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DOI：
10.1099/mic.0.28626-0
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发表时间：
2006-05-01
期刊：
影响因子：
2.8
通讯作者：
Xu, Jianping
中科院分区：
文献类型：
--
作者：
Lan, Lisa;Xu, Jianping
季也蒙念珠菌是一种单倍体条件致病菌，约占人类血液酵母菌感染的2%.最近使用多位点酶电泳和核型分析的分析表明，来自人类来源的菌株传统上指定为C。guilliermondii实际上至少包括两个种，C.季也蒙酵母和发酵假丝酵母。然而，这两个物种内部和之间的分子变异模式在很大程度上仍然未知。在这项研究中，对从加拿大、中国、菲律宾和坦桑尼亚收集的37个菌株的每一个的5个基因的DNA片段进行了测序。这些分析确定了C. guilliermondii和C.发酵五个基因系谱没有表现出明显的不一致性，表明在自然界中这两个物种的主要克隆繁殖结构。事实上，C.其中一株来自加拿大安大略省，另一株来自中国。有趣的是，结果表明，目前命名为C。guilliermondii可能含有另外的分歧谱系。在实践方面，结果显示了几个诊断分子标记，可用于临床微生物学实验室，以区分C。2006年1月17日修订guilliermondii和C.发酵这里进行的多基因系谱分析显示，在这个重要的致病性酵母菌复合体中存在显著的分歧和克隆扩散。
Candida guilliermondii is a haploid opportunistic pathogen accounting for about 2 % of human blood yeast infections. Recent analyses using multilocus enzyme electrophoresis and karyotyping suggest that strains from human sources traditionally designated C. guilliermondii in fact include at least two species, C. guilliermondii and Candida fermentati. However, the patterns of molecular variation within and between these two species remain largely unknown. In this study, DNA fragments were sequenced from five genes for each of 37 strains collected from Canada, China, the Philippines and Tanzania. The analyses identified significant sequence differences between C. guilliermondii and C. fermentati. The five gene genealogies showed no apparent incongruence, suggesting a predominantly clonal reproductive structure for both species in nature. Indeed, two large clones of C. guilliermondii were identified, with one from Ontario, Canada, and the other from China. Interestingly, the results indicate that strains currently designated C. guilliermondii may contain additional divergent lineages. On the practical side, the results revealed several diagnostic Received 24 October 2005 molecular markers that can be used in clinical microbiology laboratories to distinguish C. Revised 17 January 2006 guilliermondii and C. fermentati. The multiple gene genealogical analyses conducted here revealed Accepted 18 January 2006 significant divergence and clonal dispersal in this important pathogenic yeast complex.