The Flavobacterium psychrophilum OmpA, an outer membrane glycoprotein, induces a humoral response in rainbow trout
The Flavobacterium psychrophilum OmpA, an outer membrane glycoprotein, induces a humoral response in rainbow trout
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DOI：
10.1111/j.1365-2672.2007.03359.x
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发表时间：
2007-11-01
影响因子：
4
通讯作者：
Le Henaff, M.
中科院分区：
文献类型：
--
作者：
Dumetz, F.;LaPatra, S.-E.;Le Henaff, M.
目的：本研究的目的是表征OmpA，从嗜冷黄杆菌的膜组分分离的主要糖蛋白，并评估其作为可能的疫苗中的抗原单位的潜力。方法和结果：ompA的表达产物是465个氨基酸的蛋白质前体，其含有21个氨基酸的信号肽，并且与属于黄杆菌科的一些细菌的类似大小的蛋白质具有总体同源性（高达60%同一性）。羧基末端区域含有“OmpA / MotB”结构域/特征，并且在中心区域中已经鉴定了五个推定的“血小板反应蛋白3型重复”结构域。在外膜部分中清楚地检测到OmpA，并证明其表面暴露。OmpA是免疫显性抗原之一，特异性抗OmpA抗体的结合导致补体存在下的细胞裂解。用弗氏佐剂乳化的OmpA免疫鱼产生了较高的抗体滴度。结论：总的来说，这里获得的数据表明，OmpA可能参与嗜冷芽孢杆菌/宿主细胞的相互作用，似乎是一个潜在的疫苗免疫原。本研究的意义和影响：本研究是了解嗜冷镰刀菌致病机理和未来疫苗开发方向的一步。
Aims: The purpose of this study was to characterize OmpA, a major glycoprotein isolated from the membrane fraction of Flavobacterium psychrophilum, and to evaluate its potential as antigenic unit in a possible vaccine. Methods and Results: The expression product of ompA is a 465- amino- acid protein precursor that contains a 21- amino acid signal peptide and has overall homology ( up to 60% identity) with similarly sized proteins of some bacteria belonging to the Flavobacteriaceae family. The carboxy- terminal region contains the ' OmpA / MotB' domain / signature and five putative ' Thrombospondin type 3 repeats' domains have been identified in the central region. OmpA was clearly detected in the outer membrane fraction and its surface exposure was demonstrated. OmpA is one of the immunodominant antigens and binding of specific anti- OmpA antibodies lead to cell lysis in the presence of complement. Fish immunized with OmpA emulsified with Freund's adjuvant developed a high antibody titter. Conclusions: Collectively, the data obtained here indicate that OmpA may be involved in Fl. psychrophilum / host cell interactions and appears to be a potential immunogen for a vaccine. Significance and Impact of the Study: This study is one step in the direction of understanding pathogenesis of Fl. psychrophilum and development of future vaccine.