A quantitative study of pinocytosis and intracellular proteolysis in rat peritoneal macrophages.
A quantitative study of pinocytosis and intracellular proteolysis in rat peritoneal macrophages.
复制标题
大鼠腹膜巨噬细胞胞饮作用和细胞内蛋白水解作用的定量研究。
作者：
M. Pratten;K. E. Williams;J. Lloyd
本文介绍了一种体外培养大鼠腹膜巨噬细胞的方法。在该方法中，胶体[~(198)Au]金、~(125)I标记的聚(乙烯基吡咯烷酮)和[~(14)C]蔗糖以稳定和相当可重复性的速率进行数小时的胞质摄取。胶体[~(198)Au]金的摄取率有一定的批间变化，约为0.02%。是其他两种底物的100倍。胶体金不影响~(125)I标记的聚乙烯吡咯烷酮的摄取速率，因此其自身的高摄取速率不能归因于对胞饮小泡形成的刺激。结果表明，胶体金的摄取高度依赖于质膜上结合部位的吸附。摄取经甲醛处理的~(125)I标记牛血清白蛋白后，[~(125)I]碘-L酪氨酸释放到培养液中，并以介于胶体金和另外两种不可消化底物--~(125)I标记的聚(乙烯基吡咯烷酮)和[~(14)C]蔗糖之间的速率发生。
A method for the culture of rat peritoneal macrophages in vitro is described, in which pinocytic uptake of colloidal [198 Au]gold, 125I--labelled poly(vinylpyrrolidone) and [14C]sucrose proceeds at contant and fairly reproducible rates for several hours. The rat of uptake of colloidal [198 Au]gold, which wxhibited some inter-batch variation, was approx. 100 times that of the other two substrates. Colloidal gold did not affect the rate of uptake of 125I-labelled poly(vinylpyrrolidone) and therefore its own high rate of uptake could not be attributed to a stimulation of the formation of pinocytic vesicles. It conclude that uptake of collodial gold is highly dependent on adsorption on binding sites on the plasma membrane. Uptake of formaldehyde-treated 125I-labelled bovine serum albumin was followed by the release of [125I]iodo-L-tyrosine into the culture medium and took place at a rate intermediate between those of collodial [198Au]gold and the other two non-digestible substrates, 125I-labelled poly(vinylpyrrolidone) and [14C]sucrose.