Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation
Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation
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DOI：
10.1093/molehr/gah132
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Kirchhoff, C
中科院分区：
文献类型：
--
作者：
Obermann, H;Raabe, I;Kirchhoff, C
一种新的profilin，命名为profilin IV，被克隆和表征为睾丸亚型，不同于先前描述的睾丸特异性profilin III。Profilin IV显示与其他哺乳动物profilins只有30%的氨基酸同一性，然而，数据库搜索产生了显着的保守profilin结构域的对齐。北方印迹分析和原位转录物杂交表明，profilin IV，像profilin III，在生殖细胞中转录。然而，它们在大鼠生后睾丸发育和大鼠精子发生周期中的表达时间是不同的。在人类睾丸中，profilin IV mRNA的表达与生殖细胞的存在相关，这表明它可能是一个合适的分子诊断参数，以补充传统的组织病理学诊断评估睾丸活检。使用抗寡肽抗体验证预测的profilin IV蛋白。Western印迹分析检测到约14 kDa的免疫相关睾丸蛋白。免疫组化显示，细胞内的蛋白质的大鼠，小鼠和人类睾丸积累不对称的圆形和细长的精子细胞的细胞质中，其核周的位置与发展的顶体-顶体和柄的位置相一致。因此，Profilin IV可能调节睾丸肌动蛋白细胞骨架动力学，并在顶体生成和精子细胞核成形中发挥作用。
A novel profilin, named profilin IV, was cloned and characterized as a testicular isoform, distinct from the previously described testis-specific profilin III. Profilin IV showed only 30% amino acid identity with the other mammalian profilins; nevertheless, database searches produced significant alignments with the conserved profilin domain. Northern blot analysis and in situ transcript hybridization suggested that profilin IV, like profilin III, is transcribed in the germ cells. However, the timing of their expression during post-natal development of rat testis and in the rat spermatogenetic cycle was distinct. In the human testis, profilin IV mRNA expression correlates with the presence of germ cells suggesting that it may be a suitable molecular diagnostic parameter to supplement conventional histopathological diagnostics in the assessment of testicular biopsies. The predicted profilin IV protein was verified employing an anti-oligopeptide antibody. Western blot analysis detected an immunorelated testicular protein of approximately 14 kDa. Immunohistochemistry revealed an intracellular protein of the rat, the mouse and the human testis accumulating asymmetrically in the cytoplasm of round and elongating spermatids with its perinuclear location coinciding with the position of the developing acrosome-acroplaxome and the manchette. Profilin IV thus may regulate testicular actin cytoskeleton dynamics and play a role in acrosome generation and spermatid nuclear shaping.