A sunlight-induced rapid synthesis of silver nanoparticles using sodium salt of N-cholyl amino acids and its antimicrobial applications
A sunlight-induced rapid synthesis of silver nanoparticles using sodium salt of N-cholyl amino acids and its antimicrobial applications
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DOI：
10.1016/j.colsurfb.2012.03.009
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发表时间：
2012-08-01
影响因子：
5.8
通讯作者：
Rajendiran, N.
中科院分区：
文献类型：
--
作者：
Annadhasan, M.;SankarBabu, V. R.;Rajendiran, N.
在不需要添加稳定剂和封盖剂的情况下，用阳光照射含有硝酸银（AgNO3）和n -酰氨基酸钠盐两种添加剂的水溶液，合成了球形AgNPs。n -胆酰氨基酸浓度的变化对AgNPs的形态有很好的控制，而胆汁盐的羧酸基减少了Ag+离子，酰胺基与NPs表面结合强烈。通过紫外可见、透射电镜（TEM）和动态光散射（DLS）研究了AgNPs的光学性质和形貌。利用循环伏安法和傅里叶变换红外光谱技术研究了n -羧基氨基酸与AgNPs表面的相互作用。还原过程在5分钟内完成，合成的AgNPs稳定性超过6个月。讨论了n -巯基氨基酸对AgNPs还原和稳定的可能机理。根据CLSI指南，采用MIC方法测定了n -羧基氨基酸包盖AgNPs对Mueller Hinton肉汤中大肠埃希菌、金黄色葡萄球菌和气产假单胞菌的抑菌活性，以及RPMI肉汤中对白色念珠菌、克鲁氏念珠菌和热带念珠菌的抑菌活性。C) 2012 Elsevier B.V.版权所有
Aqueous solution containing two additives, silver nitrate (AgNO3) and sodium salt of N-cholyl amino acid were irradiated by sunlight for the synthesis of spherical shaped AgNPs without the need for an additional stabilizer or capping agent. Variations of N-cholyl amino acid concentration provided good control over the morphology of the AgNPs, while the carboxylate group of bile salt reduced the Ag+ ions and the amide group binds strongly to the surface of the NPs. The optical properties, morphology of the AgNPs were characterized by UV-visible, transmission electron microscopy (TEM) and dynamic light scattering (DLS) studies. The interaction of N-cholyl amino acid on the AgNPs surface was studied using cyclic voltammetry and FT-IR techniques. The reduction process was completed within 5 min and the synthesized AgNPs were stable for more than 6 months. The possible mechanism of N-cholyl amino acid on the reduction and stabilization of AgNPs is also discussed. The antimicrobial activity of N-cholyl amino acid capped AgNPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginosa using Mueller Hinton broth and the antifungal activity against Candida albicans, Candida krusei and Candida tropicalis using RPMI broth were determined by MIC studies as per CLSI guidelines. C) 2012 Elsevier B.V. All rights reserved.