A Novel RNA-Binding Protein Involves ABA Signaling by Post-transcriptionally Repressing ABI2.
A Novel RNA-Binding Protein Involves ABA Signaling by Post-transcriptionally Repressing ABI2.
复制标题
一种新型 RNA 结合蛋白通过转录后抑制 ABI2 参与 ABA 信号传导。
DOI：
10.3389/fpls.2017.00024
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Xu J;Chen Y;Qian L;Mu R;Yuan X;Fang H;Huang X;Xu E;Zhang H;Huang J
受阿坝、盐和冷抑制的应激相关RNA结合蛋白1（SRP1）编码拟南芥中的C2 C2型锌指蛋白。srp1基因的敲除突变降低了种子在萌发和萌发后生长阶段对阿坝和盐胁迫的敏感性。相反，SRP1过表达幼苗比野生型植物对阿坝和盐更敏感。在阿坝的存在下，阿坝信号转导和萌发相关基因包括ABI 3. ABI 5。与WT相比，EM 1和EM 6在srp 1中诱导较少。有趣的是，编码蛋白磷酸酶2C蛋白的ABI2的表达在srp1突变体中显著上调。体外分析表明，SRP1是一种新的RNA结合蛋白，可与ABI2 mRNA的3′UTR直接结合。瞬时表达实验证实了SRP1对ABI2 3'UTR融合的荧光素酶具有抑制作用。总之，这表明SRP1参与阿坝信号转导转录后抑制ABI2在拟南芥中的表达。
The Stress Associated RNA-binding protein 1 (SRP1) repressed by ABA, salt and cold encodes a C2C2-type zinc finger protein in Arabidopsis. The knock-out mutation in srp1 reduced the sensitivity of seed to ABA and salt stress during germination and post-germinative growth stages. In contrast, SRP1-overexpressing seedlings were more sensitive to ABA and salt compared to wild type plants. In the presence of ABA, the transcript levels of ABA signaling and germination-related genes including ABI3. ABI5. EM1 and EM6 were less induced in srp1 compared to WT. Interestingly, expression of ABI2 encoding a protein phosphatase 2C protein were significantly up-regulated in srp1 mutants. By in vitro analysis, SRP1 was identified as a novel RNA-binding protein directly binding to 3′UTR of ABI2 mRNA. Moreover, transient expression assay proved the function of SRP1 in reducing the activity of luciferase whose coding sequence was fused with the ABI2 3’UTR. Together, it is suggested that SRP1 is involved in the ABA signaling by post-transcriptionally repressing ABI2 expression in Arabidopsis.