alpha(2)-adrenergic receptor subtypes in rat dorsal root and superior cervical ganglion neurons
alpha(2)-adrenergic receptor subtypes in rat dorsal root and superior cervical ganglion neurons
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DOI：
10.1016/s0304-3959(96)03218-6
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发表时间：
1997-01-01
期刊：
影响因子：
7.4
通讯作者：
Levine, JD
中科院分区：
文献类型：
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作者：
Gold, MS;Dastmalchi, S;Levine, JD
为了确定初级传入和交感神经节后神经元中存在哪些α(2)-肾上腺素能受体亚型，我们对大鼠背根和颈上神经节进行了原位杂交和免疫组织化学实验。逆转录-聚合酶链式反应初步筛选α(2)-肾上腺素能亚型在背根神经节和颈上神经节的表达；聚合酶链式反应扩增大鼠腰背根和颈上神经节mRNA中α(2A)-(RG20)、α(2B)-(RNG)和α(2C)-(RG10)受体亚型的不同区域，以定位神经节细胞类型的受体，用设计用于区分α(2)-受体亚型的寡核苷酸探针对背根节和颈上神经节的冰冻切片进行原位杂交，用针对α(2A)-肾上腺素能受体亚型的多克隆抗体进行免疫组织化学，逆转录-聚合酶链式反应结果表明，三种α(2)-肾上腺素能受体亚型均表达于背根神经节和颈上神经节。原位杂交结果表明，逆转录-聚合酶链式反应检测到的mRNA存在于神经元胞体中，但编码α(2A)-肾上腺素能受体的mRNA在背根神经节中未检测到。编码α(2B)和α(2C)受体亚型的mRNA在背根神经节神经元中的分布以及α(2A)、α(2B)和α(2C)受体亚型在颈上神经节神经元中的分布表明，单个神经元中存在多种肾上腺素能受体亚型。背根神经节和颈上神经节的神经元胞体均呈A型肾上腺素能受体样免疫反应。多个α(2)-肾上腺素能受体亚型在背根和颈上神经节神经元中的明显共表达使单个递质在同一神经元中产生多种作用；哪些受体功能活跃可能会因神经损伤、炎症或其他生理和病理生理条件的存在而有所不同。
To determine which alpha(2)-adrenergic receptor subtypes are present in primary afferent and sympathetic postganglionic neurons we have performed in situ hybridization and immunohistochemistry experiments on rat dorsal root and superior cervical ganglia. Reverse transcriptase polymerase chain reaction was used as a preliminary screen for the presence of mRNA encoding alpha(2)-adrenergic subtypes in dorsal root and superior cervical ganglia; polymerase chain reaction primers amplified distinct regions of the rat alpha(2A)-(RG20), alpha(2B)-(RNG) and alpha(2C)-(RG10) adrenergic receptor subtypes in mRNA extracted from lumbar dorsal root and superior cervical ganglia, To localize receptors to cell types in the ganglia, in situ hybridization was performed on cryosections of dorsal root and superior cervical ganglia with oligonucleotide probes designed to distinguish between mRNA encoding for alpha(2)-adrenergic receptor subtypes, Immunohistochemistry was performed with a polyclonal antibody against the alpha(2A)-adrenergic receptor subtype, Our results with reverse transcriptase polymerase chain reaction indicate that all three alpha(2)-adrenergic receptor subtypes are expressed in dorsal root and superior cervical ganglia. Data from the in situ hybridization experiments indicated that the mRNA detected with the reverse transcriptase polymerase chain reaction was present in neuronal cell bodies, except for the mRNA encoding the alpha(2A)-adrenergic receptor which was not detectable in dorsal root ganglia. The distribution of mRNA encoding alpha(2B)- and alpha(2C)-adrenergic receptor subtypes among dorsal root ganglion neurons and alpha(2A)-, alpha(2B)- and alpha(2C)-adrenergic receptor subtypes among superior cervical ganglion neurons suggests that multiple adrenergic receptor subtypes are present in a single neuron. Neuronal cell bodies in both the dorsal root and superior cervical ganglion consistently demonstrated aw-adrenergic receptor-like immunoreactivity. The apparent co-expression of multiple alpha(2)-adrenergic receptor subtypes in dorsal root and superior cervical ganglion neurons enables a single transmitter to produce a number of effects in the same neuron; which receptors are functionally active may vary with the presence of nerve injury, inflammation or other physiological and pathophysiological conditions.