Molecular cloning and functional identification of an insect odorant receptor gene in Spodoptera litura (F.) for the botanical insecticide rhodojaponin III
Molecular cloning and functional identification of an insect odorant receptor gene in Spodoptera litura (F.) for the botanical insecticide rhodojaponin III
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斜纹夜蛾昆虫气味受体基因的分子克隆及功能鉴定，用于植物源杀虫剂红粳甙III
DOI：
10.1016/j.jinsphys.2012.11.004
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发表时间：
2013-01-01
影响因子：
2.2
通讯作者：
Wang, Huidong
中科院分区：
文献类型：
--
作者：
Dong, Xiaolin;Zhong, Guohua;Wang, Huidong
昆虫化学感受器上的感受器识别外界环境中的化学物质，从而刺激其特定的行为。闹羊花素Ⅲ是一种非挥发性天然植物杀虫剂，对多种昆虫有强烈的产卵忌避作用。本研究旨在确定Or 83 b蛋白是否参与识别杜鹃素III。SlOr 83 b蛋白具有保守的七跨膜结构域和推定的受体/受体相互作用环。Or 83 b mRNA首先在第1期幼虫中转录，并在后期幼虫阶段表达。Or 83 b mRNA在蛹期和成虫期均有高水平表达。将双链RNA（dsRNA）注射到第8天的蛹中，通过RNAi实现了SlOr 83 b的表达的敲低，并且在杜鹃花素III处理的甘蓝叶片上产卵显著增加。这些结果表明，Or 83 b是昆虫识别非挥发性杀虫剂杜鹃花素III的关键。皇冠版权所有（C）2012由爱思唯尔有限公司出版。保留所有权利。
Specific behaviors of insects are stimulated by the recognition of chemicals in the external environment by sensilla on chemoreceptive organs. Rhodojaponin III is a nonvolatile natural botanical insecticide, which has an intense effect of deterring oviposition activity against many insects. This study aimed to determine whether the Or83b protein is involved in the recognition of rhodojaponin III. The SlOr83b protein has a conserved seven-transmembrane domain and a putative receptor/receptor interaction loop. The Or83b mRNA is first transcribed in 1st larvae, and expressed in later larval stages. A high level of Or83b mRNA is expressed in pupal and adult stages. Knockdown of SlOr83b expression was achieved through RNAi by injecting double-stranded RNA (dsRNA) into the 8th day pupae, and oviposition significantly increased on rhodojaponin III treated cabbage leaves. These results suggest that Or83b is critical for insects to identify the nonvolatile the insecticide rhodojaponin III. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.