Primary leaf-type ferredoxin 1 participates in photosynthetic electron transport and carbon assimilation in rice
Primary leaf-type ferredoxin 1 participates in photosynthetic electron transport and carbon assimilation in rice
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DOI：
10.1111/tpj.14904
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发表时间：
2020-08-12
期刊：
影响因子：
7.2
通讯作者：
Zhu, Li
中科院分区：
文献类型：
--
作者：
He, Lei;Li, Man;Zhu, Li
铁氧还蛋白（Ferredoxins，Fds）通过调节电子向下游酶的分配在光合作用中起着至关重要的作用。在水稻（Oryza sativa L.）（水稻）基因组;然而，他们的具体功能还没有得到很好的理解。在这里，我们报告riceFd 1的功能特性。序列比对、系统发育分析和定量逆转录聚合酶链反应（qRT-PCR）分析表明，水稻Fd 1为初生叶型Fd。NADP（+）和细胞色素c的电子转移实验表明，Fd 1可以从光系统I（PSI）向铁氧还蛋白-NADP（+）还原酶提供电子。fd 1功能丧失突变体在三叶期表现出失绿和幼苗致死。Fd 1的缺乏会损害光合电子传递，从而影响碳同化。外源葡萄糖处理部分恢复了突变体表型，表明Fd 1在水稻光合电子传递中起重要作用。另外，在infd 1突变体中，Fd依赖基因的转录水平也受到影响，其变化趋势与观察到的infdc 2植株相似。这些结果表明OsFd 1是水稻光合电子传递和碳同化的主要Fd。
Ferredoxins (Fds) play a crucial role in photosynthesis by regulating the distribution of electrons to downstream enzymes. MultipleFdgenes have been annotated in theOryza sativa L. (rice) genome; however, their specific functions are not well understood. Here, we report the functional characterization of riceFd1. Sequence alignment, phylogenetic analysis of seven rice Fd proteins and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that rice Fd1 is a primary leaf-type Fd. Electron transfer assays involving NADP(+)and cytochrome cindicated that Fd1 can donate electrons from photosystem I (PSI) to ferredoxin-NADP(+)reductase. Loss-of-functionfd1mutants showed chlorosis and seedling lethality at the three-leaf stage. The deficiency of Fd1 impaired photosynthetic electron transport, which affected carbon assimilation. Exogenous glucose treatment partially restored the mutant phenotype, suggesting that Fd1 plays an important role in photosynthetic electron transport in rice. In addition, the transcript levels of Fd-dependent genes were affected infd1mutants, and the trend was similar to that observed infdc2plants. Together, these results suggest that OsFd1 is the primary Fd in photosynthetic electron transport and carbon assimilation in rice.