Major secretory protein of human decidualized endometrium in pregnancy is an insulin-like growth factor-binding protein.
Major secretory protein of human decidualized endometrium in pregnancy is an insulin-like growth factor-binding protein.
复制标题
妊娠期人蜕膜化子宫内膜的主要分泌蛋白是胰岛素样生长因子结合蛋白。
作者：
S. Bell;S. Patel;J. Jackson;G. Waites
妊娠相关子宫内膜α 1-球蛋白（α 1-PEG）是妊娠期间人类蜕膜化子宫内膜在体外合成和分泌的主要分泌蛋白产物。这种蛋白质已从该组织的胞质提取物中纯化，现在已被表征为32 kDa的生长调节素/胰岛素样生长因子（IGF）结合蛋白。从羊水中分离的免疫反应性α 1-PEG表现出相同的理化性质和IGF-I结合特征。在妊娠子宫内膜的胞质提取物中，在该组织的孵育介质和羊水中，32 kDa的蛋白质代表主要的α 1-PEG免疫反应性蛋白和主要的IGF-I结合组分。纯化的α 1-PEG和妊娠子宫内膜孵育液在体外与胎盘膜IGF受体竞争IGF-I。参考羊水和血清小先生IGF-结合蛋白的起源和建议的旁分泌对滋养层细胞增殖的影响，IGF-I结合蛋白的子宫内膜来源的影响进行了讨论。
Pregnancy-associated endometrial alpha 1-globulin (alpha 1-PEG) is quantitatively the major secretory protein product, synthesized and secreted in vitro, of the human decidualized endometrium during pregnancy. This protein has been purified from cytosolic extracts of this tissue and has now been characterized as a 32 kDa somatomedin/insulin-like growth factor (IGF)-binding protein. Immunoreactive alpha 1-PEG isolated from amniotic fluid exhibited identical physiochemical properties and IGF-I-binding characteristics. In cytosolic extracts of pregnancy endometrium, in incubation medium of this tissue and in amniotic fluid, the 32 kDa protein represented the major alpha 1-PEG immunoreactive protein and major IGF-I-binding component. Purified alpha 1-PEG and incubation medium of pregnancy endometrium competed for IGF-I with placental membrane IGF receptors in vitro. The implications of the endometrial source of IGF-I-binding protein are discussed with reference to the origin of the amniotic fluid and serum small Mr IGF-binding protein and to the suggested paracrine effect upon trophoblast proliferation.