Protective immunity by intramuscular injection of low doses of influenza virus DNA vaccines.
Protective immunity by intramuscular injection of low doses of influenza virus DNA vaccines.
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DOI：
10.1016/0264-410x(94)90081-7
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发表时间：
1994-12
期刊：
影响因子：
5.5
通讯作者：
J. Ulmer;R. Deck;C. DeWITT;A. Friedman;J. Donnelly;Margaret A. Liu
中科院分区：
文献类型：
--
作者：
J. Ulmer;R. Deck;C. DeWITT;A. Friedman;J. Donnelly;Margaret A. Liu
研究了流感病毒血凝素 (HA) 或核蛋白 (NP) DNA 疫苗的剂量与基于体液和细胞免疫的免疫原性和保护功效之间的剂量反应关系。在小鼠中，肌内 (i.m.) 注射 100 ng 至 1 μg 剂量的 HA 或 NP DNA，分别产生血凝抑制 (HI) 抗体和细胞毒性 T 淋巴细胞，并在流感病毒攻击模型中提供保护。观察到注射的 DNA 量与 HI 抗体水平之间存在直接相关性。在非人类灵长类动物中，接种低至 10 μg HA DNA 的动物即可诱导产生高滴度中和抗体。这些结果表明，肌内注射低剂量的 DNA。注射液具有预防流感的功效。
Dose—response relationships were investigated between dose of influenza virus haemagglutinin (HA) or nucleoprotein (NP) DNA vaccines, and immunogenicity and protective efficacy based on humoral and cellular immunity. In mice, intramuscular (i.m.) injection of HA or NP DNA, at doses of 100 ng to 1 μg, was found to generate haemagglutination inhibiting (HI) antibodies and cytotoxic T-lymphocytes, respectively, and provide protection in influenza virus challenge models. A direct correlation between the amount of DNA injected and the level of HI antibody was observed. In non-human primates, high-titre neutralizing antibodies were induced in animals vaccinated with as little as 10 μg of HA DNA. These results indicate that low doses of DNA administered by i.m. injection provide protective efficacy against influenza.