SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells.
SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells.
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SV40内含子，一种有效的强内含子元件，可有效增加转染的中国仓鼠卵巢细胞中的转基因表达
DOI：
10.1111/jcmm.13504
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发表时间：
2018-04
影响因子：
5.3
通讯作者：
Zhang YN
中科院分区：
文献类型：
--
作者：
Xu DH;Wang XY;Jia YL;Wang TY;Tian ZW;Feng X;Zhang YN
Chinese hamster ovary (CHO) cells have become the most widely utilized mammalian cell line for the production of recombinant proteins. However, the product yield and transgene instability need to be further increased and solved. In this study, we investigated the effect of five different introns on transgene expression in CHO cells. hCMV intron A, adenovirus tripartite leader sequence intron, SV40 intron, Chinese hamster EF‐1alpha gene intron 1 and intervening sequence intron were cloned downstream of the eGFP expression cassette in a eukaryotic vector, which was then transfected into CHO cells. qRT‐PCR and flow cytometry were used to explore eGFP expression levels. And gene copy number was also detected by qPCR, respectively. Furthermore, the erythropoietin (EPO) protein was used to test the selected more strong intron. The results showed that SV40 intron exhibited the highest transgene expression level among the five compared intron elements under transient and stable transfections. In addition, the SV40 intron element can increase the ratio of positive colonies and decrease the coefficient of variation in transgene expression level. Moreover, the transgene expression level was not related to the gene copy number in stable transfected CHO cells. Also, the SV40 intron induced higher level of EPO expression than IVS intron in transfected CHO cell. In conclusion, SV40 intron is a potent strong intron element that increases transgene expression, which can readily be used to more efficient transgenic protein production in CHO cells.
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影响因子：
3.5
作者：
Johansen, J;Tornoe, J;Johansen, TE
通讯作者：
Johansen, TE
DOI：
10.1038/mtna.2013.47
发表时间：
2013-09-03
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.7
作者：
Kang, Shin-Young;Kim, Yeon-Gu;Kang, Seunghee;Lee, Hong Weon;Lee, Eun Gyo
通讯作者：
Lee, Eun Gyo
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影响因子：
3.5
作者：
Lin, Yan;Li, Zhaoxi;Yang, Xianjun
通讯作者：
Yang, Xianjun
影响因子：
16.8
作者：
Gudikote, JP;Imam, JS;Wilkinson, MF
通讯作者：
Wilkinson, MF