Identification of microRNA-like RNAs in a plant pathogenic fungus Sclerotinia sclerotiorum by high-throughput sequencing
Identification of microRNA-like RNAs in a plant pathogenic fungus Sclerotinia sclerotiorum by high-throughput sequencing
复制标题
高通量测序鉴定植物病原真菌核盘菌中的 microRNA 样 RNA
DOI：
10.1007/s00438-012-0678-8
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Cheng, Jiasen
中科院分区：
文献类型：
--
作者：
Zhou, Jiahong;Fu, Yanping;Cheng, Jiasen
MicroRNA是一类长度约为22 nt的非编码小RNA，在基因表达的转录后调控中具有重要的调控作用。迄今为止，在植物病原真菌中还没有miRNA的报道。在这里，我们使用Solexa测序在植物病原真菌核盘菌中发现新的miRNAs。共获得13，229，401个高质量的读段，共将5，645，718个读段定位到S.核盘菌基因组对应于348，108个独特的sRNA。在sRNA中，通过序列分析鉴定了2种microRNA-like RNA（milRNA）和42种mil-RNA候选物。北方印迹和RT-PCR结果显示，一些milRNA和候选物在菌核发育中差异表达，表明这些milRNA和候选物在该生物学过程中的作用。我们的研究结果为小RNA的功能作用提供了新的见解，并为植物病原真菌的研究增加了新的资源。
MicroRNAs are small non-coding RNAs of approximately 22 nt with important regulatory roles in post-transcriptional regulation of gene expression. To date, no miRNAs have been reported in plant pathogenic fungi. Here we used Solexa sequencing to find new miRNAs in a plant pathogenic fungus Sclerotinia sclerotiorum. 13,229,401 high-quality reads were obtained, a total of 5,645,718 reads were mapped to the S. sclerotiorum genome corresponding to 348,108 unique sRNAs. Among the sRNAs, 2 microRNA-like RNAs (milRNAs) and 42 mil-RNA candidates were identified by sequence analysis. Northern blot and RT-PCR results showed that some milRNAs and candidates are differentially expressed in sclerotial development suggesting the role of these milRNAs and candidates in this biological process. Our results provide new insights into the functional roles of small RNAs and add new resources for the study of plant pathogenic fungi.