Localization and quantitation of human superoxide dismutase using computerized 2-D gel electrophoresis.
Localization and quantitation of human superoxide dismutase using computerized 2-D gel electrophoresis.
复制标题
使用计算机二维凝胶电泳对人超氧化物歧化酶进行定位和定量。
DOI：
10.1016/s0006-291x(81)80185-4
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
Darlington,GJ
中科院分区：
文献类型：
--
作者：
Brown,WT;Dutkowski,R;Darlington,GJ
人超氧化物歧化酶的位置已确定的2-D凝胶培养的成纤维细胞提取物。人超氧化物歧化酶位于2-D凝胶的小鼠-人的体细胞杂交只含有人类21号染色体，在小鼠亲本细胞系中是不存在的。天然酶是由2-D凝胶上的两个相同单体组成的二聚体，分子量约为16，000，等电点为pH 6.2。比较21三体细胞株与21单体细胞株凝胶上的SOD斑点密度，显示3倍增加，表明存在基因剂量效应。这种方法证明了基因剂量研究的定量2-0分析的实用性。
The location of human superoxide dismutase has been identified on 2-D gels of cultured fibroblast extracts. Human superoxide dismutase was located on 2-D gels of a mouse-human somatic cell hybrid containing only human chromosome 21, and was absent in the mouse parental cell line. The native enzyme is a diner composed of two identical monomers present on 2-D gels as a single spot of molecular weight approximately 16,000 and isoelectric point of pH 6.2. Comparison of the density of the SOD spot on gels of a trisomy 21 cell strain to that of a monosomy 21 cell strain showed a 3 fold increase indicating agene dosage effect was present. This approach demonstrates the utility of quantitative 2-0 analysis for gene dosage studies.