Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles
Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles
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DOI：
10.1002/mrm.10585
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发表时间：
2003-10-01
影响因子：
3.3
通讯作者：
Syková, E
中科院分区：
文献类型：
--
作者：
Jendelová, P;Herynek, V;Syková, E
骨髓基质细胞（MSCs）是多能祖细胞，具有向病变迁移的能力，并诱导或促进响应环境信号的位点依赖性分化。在具有皮质光化学损伤的动物中，研究了用磁性氧化铁纳米颗粒（Endorem（（R）和溴脱氧尿苷（BrdU）共标记的大鼠MSC的命运。将MSC脑内移植到成年大鼠脑的对侧半球或静脉内注射。体内MRI被用来追踪它们的命运;普鲁士蓝染色和透射电子显微镜（TEM）证实了氧化铁纳米颗粒在细胞内的存在。在移植后的第一周，移植的细胞迁移到病变部位，并填充受损皮质组织的边缘区。植入的细胞在MR图像上可见，在注射部位和病变中为低信号区。低信号持续了50多天。随后的组织学染色证实存在BrdU阳性和含铁细胞。注射后3 - 4周，
Bone marrow stromal cells (MSCs) are pluripotent progenitor cells that have the capacity to migrate toward lesions and induce or facilitate site-dependent differentiation in response to environmental signals. In animals with a cortical photochemical lesion, the fate of rat MSCs colabeled with magnetic iron-oxide nanoparticles (Endorem((R))) and bromodeoxyuridine (BrdU) was studied. MSCs were either grafted intracerebrally into the contralateral hemisphere of adult rat brain or injected intravenously. In vivo MRI was used to track their fate; Prussian blue staining and transmission electron microscopy (TEM) confirmed the presence of iron-oxide nanoparticles inside the cells. During the first week posttransplantation, the transplanted cells migrated to the lesion site and populated the border zone of the damaged cortical tissue. The implanted cells were visible on MR images as a hypointense area at the injection site and in the lesion. The hypointense signal persisted for more than 50 days. The presence of BrdU-positive and iron-containing cells was confirmed by subsequent histological staining. Three to 4 weeks after injection,