Zygotic lethals with specific maternal effect phenotypes in Drosophila melanogaster. I. Loci on the X chromosome.
Zygotic lethals with specific maternal effect phenotypes in Drosophila melanogaster. I. Loci on the X chromosome.
复制标题
DOI：
--
复制
发表时间：
1989-02
期刊：
影响因子：
3.3
通讯作者：
Norbert Perrimon;L. Engstrom;A. Mahowald
中科院分区：
文献类型：
--
作者：
Norbert Perrimon;L. Engstrom;A. Mahowald
为了确定X连锁合子致死基因座对胚胎发育具有特定母体效应，对X连锁合子致死突变进行了种系克隆分析。本研究采用了两种策略。在筛选中，对先前定位的确定区域内的211个X连锁基因座上的441个突变进行了种系克隆分析。在B屏幕中，对分布在X染色体全长的581个幼虫和幼虫突变进行了种系克隆分析。这些方法为具有特定母体效应胚胎致死表型的X连锁晚期合子致死(幼虫和幼虫)提供了86%的饱和水平。描述了这些突变的母体效应表型。
In order to identify all X-linked zygotic lethal loci that exhibit a specific maternal effect on embryonic development, germline clonal analyses of X-linked zygotic lethal mutations have been performed. Two strategies were employed. In Screen A germline clonal analysis of 441 mutations at 211 previously mapped X-linked loci within defined regions was performed. In Screen B germline clonal analysis of 581 larval and pupal mutations distributed throughout the entire length of the X chromosome was performed. These approaches provide an 86% level of saturation for X-linked late zygotic lethals (larval and pupal) with specific maternal effect embryonic lethal phenotypes. The maternal effect phenotypes of these mutations are described.