Multiple sclerosis is associated with high levels of circulating dendritic cells secreting pro-inflammatory cytokines
Multiple sclerosis is associated with high levels of circulating dendritic cells secreting pro-inflammatory cytokines
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DOI：
10.1016/s0165-5728(99)00106-x
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发表时间：
1999-09-01
影响因子：
3.3
通讯作者：
Link, H
中科院分区：
文献类型：
--
作者：
Huang, YM;Xiao, BG;Link, H
最近的证据强调树突状细胞（DC）通过启动和耐受T细胞在免疫控制中的关键作用。DC捕获和加工抗原，表达共刺激分子，迁移到淋巴器官并分泌细胞因子来启动免疫反应。在多发性硬化症（multiple sclerosis， MS）中，自体反应性T细胞被认为通过分泌促炎细胞因子发挥致病作用，但对DC的研究较少。为了评估DC在多发性硬化症患者中的作用，采用一种改进的方法从多发性硬化症患者和健康受试者的血液中制备DC。发现DC在同种异体中是T细胞的有效刺激物，在较小程度上，在自体混合白细胞反应（MLR）中也是。采用酶联免疫斑点法（ELISPOT）测定MS和健康受试者中分泌ifn - γ、tnf - α、IL-6和IL-IO的DC与单核细胞（MNC）的比值。MS患者的ifn - γ、tnf - α和IL-6分泌DC水平高于健康受试者。ifn - γ和tnf - α分泌细胞的差异仅限于未治疗的MS患者亚组，而在持续使用ifn - β治疗的亚组中未观察到。循环DC分泌促炎细胞因子可能是ms免疫发病机制和治疗干预研究的另一个焦点(C) 1999 Elsevier Science b.v.版权所有。
Recent evidence emphasises a pivotal role for dendritic cells (DC)in the control of immunity by priming and tolerising T cells. DC capture and process antigens, express co-stimulatory molecules, migrate to lymphoid organs and secrete cytokines to initiate immune responses. En multiple sclerosis (MS), autoreactive T cells are proposed to play a pathogenic role by secreting pro-inflammatory cytokines, but studies on DC are lacking. To evaluate the involvement of DC in patients with MS, a modified procedure was used to prepare DC from blood of patients with MS and healthy subjects. DC were found to be potent stimulators of T cells in allogeneic and, to a lesser extent, in autologous mixed leukocyte reaction (MLR). Enzyme-linked immunospot (ELISPOT) assays were adopted to determine levels of IFN-gamma, TNF-alpha , IL-6 and IL-IO secreting DC vs, mononuclear cells(MNC) Proportionally more DC than MNC secreted IFN-gamma and IL-10 in both MS and healthy subjects. Patients with MS had higher levels of IFN-gamma, TNF-alpha and IL-6 secreting DC than healthy subjects. The differences for IFN-gamma and TNF-alpha secreting cells were confined to the subgroup of untreated MS patients and not observed in the subgroup examined during ongoing treatment with IFN-beta. Circulating DC secreting pro-inflammatory cytokines may represent another focus for the study of both immuno-pathogenesis and therapeutic interventions in MS. (C) 1999 Elsevier Science B.V All rights reserved.