Triggered release of insulin from glucose-sensitive enzyme multilayer shells.
Triggered release of insulin from glucose-sensitive enzyme multilayer shells.
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DOI：
10.1016/j.biomaterials.2009.01.027
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发表时间：
2009-05
期刊：
影响因子：
14
通讯作者：
W. Qi;Xuehai Yan;J. Fei;Anhe Wang;Yue Cui;Junbai Li
中科院分区：
文献类型：
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作者：
W. Qi;Xuehai Yan;J. Fei;Anhe Wang;Yue Cui;Junbai Li
采用层层组装法制备了一种葡萄糖敏感的多层壳，可作为胰岛素的包封和控释载体。本文采用戊二醛交联法将葡萄糖氧化酶（GOD）和过氧化氢酶（CAT）交替组装在胰岛素颗粒上。所得核-壳系统已被证明是葡萄糖敏感的。当外加葡萄糖时，胰岛素从蛋白质多层膜中的释放率显著增加。这可能归因于CAT/GOD壳与葡萄糖的催化相互作用，其导致H+的产生，从而降低微环境的pH。在酸性条件下，一方面，由席夫碱反应形成的CN键的一部分可以被打破，从而增加胶囊壁的渗透性。另一方面，还可以增加胰岛素的溶解度。上述因素可能是增加胰岛素从多层膜中释放的关键控制因素。因此，这种CAT/GOD多层膜作为胰岛素的葡萄糖敏感性释放载体具有很大的潜力，并为LbL胶囊在药物释放和控制释放等方面的进一步应用开辟了道路。
A glucose-sensitive multilayer shell, which was fabricated by the layer-by-layer (LbL) assembly method, can be used as a carrier for the encapsulation and controlled release of insulin. In the present report, glucose oxidase (GOD) and catalase (CAT) were assembled on insulin particles alternately via glutaraldehyde (GA) cross-linking. The resulting core–shell system has been proven to be glucose-sensitive. When the external glucose was introduced, the release ratio of insulin from the protein multilayer can be increased observably. This is likely attributed to the catalysis interaction of CAT/GOD shells to glucose, which leads to the production of H+and thus drops the pH of the microenvironment. Under the acidic conditions, on the one hand, a part of CN bond formed from Schiff base reaction can be broken and thus increasing the permeability of the capsule wall. On the other hand, the solubility of insulin can also be increased. The above factors may be the key control to increase the release of insulin from the multilayer. Therefore, such CAT/GOD multilayer may have a great potential as a glucose-sensitive release carrier for insulin, and may open the way for the further application of LbL capsules in the drug delivery and controlled release, etc.