Oxygen consumption and body temperature of active and resting honeybees.
Oxygen consumption and body temperature of active and resting honeybees.
复制标题
活跃和休息蜜蜂的耗氧量和体温。
DOI：
10.1016/s0022-1910(03)00148-3
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发表时间：
2003
影响因子：
2.2
通讯作者：
K. Crailsheim
中科院分区：
文献类型：
--
作者：
A. Stabentheiner;J. Vollmann;H. Kovac;K. Crailsheim
我们测量的能量周转（耗氧量）的蜜蜂（意大利蜜蜂carnica），这是自由移动的瓦尔堡船只。活跃蜜蜂的耗氧量随环境温度和活动水平的不同而变化很大，但觅食蜜蜂（>18 d）和中年蜜蜂（7-10 d）之间没有差异。在处于吸热状态准备飞行的高度活跃的蜜蜂中，它几乎呈线性下降，从15 °C环境温度下的最大值131.4 μl O2 min − 1下降到25 °C时的81.1 μl min− 1，并在40 °C时达到最小值29.9 μl min− 1。在低活动的蜜蜂中，它从15 °C时的89.3 μl O2 min − 1下降到25 °C时的47.9 μl min− 1和40 °C时的14.7 μl min− 1。体温的热成像测量表明，随着活动的增加，蜜蜂投入更多的能量来调节胸部温度，使其达到越来越高的水平（高度活跃的蜜蜂为38.8-41.2 °C），并且更加准确。在1-7小时龄的幼蜂中测定了静息代谢，这些幼蜂还不能用它们的飞行肌肉产生吸热热。它们的能量周转从10 °C时的0.21 μl O2 min − 1增加到15 °C时的0.38 μl min− 1，25 °C时的1.12 μl min− 1和40 °C时的3.03 μl min− 1。在15、25和40 °C下，这分别是高活性蜜蜂的343、73和10倍。而休眠蜜蜂的Q10值随环境温度的变化呈U型变化，而非恒定值。它从11-21 °C温度范围内的4.24下降到21-31 °C范围内的1.35，并在30-40 °C范围内再次增加到2.49。我们的结论是，试图描述的温度依赖性的蜜蜂的静息代谢的Q10值可能会导致相当大的误差，如果测量仅在两个温度下进行。根据指数函数VO 2 =0.151×1.0784Ta（内插Q10=2.12）计算内插Q10，可以得到可接受的近似值。
We measured the energy turnover (oxygen consumption) of honeybees (Apis mellifera carnica), which were free to move within Warburg vessels. Oxygen consumption of active bees varied widely depending on ambient temperature and level of activity, but did not differ between foragers (>18 d) and middle-aged hive bees (7–10 d). In highly active bees, which were in an endothermic state ready for flight, it decreased almost linearly, from a maximum of 131.4 μl O2min−1at 15 °C ambient temperature to 81.1 μl min−1at 25 °C, and reached a minimum of 29.9 μl min−1at 40 °C. In bees with low activity, it decreased from 89.3 μl O2min−1at 15 °C to 47.9 μl min−1at 25 °C and 14.7 μl min−1at 40 °C. Thermographic measurements of body temperature showed that with increasing activity, the bees invested more energy to regulate the thorax temperature at increasingly higher levels (38.8–41.2 °C in highly active bees) and were more accurate. Resting metabolism was determined in young bees of 1–7 h age, which are not yet capable of endothermic heat production with their flight muscles. Their energy turnover increased from 0.21 μl O2min−1at 10 °C to 0.38 μl min−1at 15 °C, 1.12 μl min−1at 25 °C, and 3.03 μl min−1at 40 °C. At 15, 25 and 40 °C, this was 343, 73 and 10 times below the values of the highly active bees, respectively. The Q10value of the resting bees, however, was not constant but varied in a U-shaped manner with ambient temperature. It decreased from 4.24 in the temperature range 11–21 °C to 1.35 in the range 21–31 °C, and increased again to 2.49 in the range 30–40 °C. We conclude that attempts to describe the temperature dependence of the resting metabolism of honeybees by Q10values can lead to considerable errors if the measurements are performed at only two temperatures. An acceptable approximation can be derived by calculation of an interpolated Q10according to the exponential functionVO2=0.151×1.0784Ta(interpolated Q10=2.12).