Bisphenol A-induced apoptosis of cultured rat Sertoli cells
Bisphenol A-induced apoptosis of cultured rat Sertoli cells
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DOI：
10.1016/s0890-6238(03)00034-0
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发表时间：
2003-07-01
影响因子：
3.3
通讯作者：
Yamada, F
中科院分区：
文献类型：
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作者：
Iida, H;Maehara, K;Yamada, F
研究了双酚A(BPA)对培养的18日龄大鼠睾丸支持细胞的影响。我们发现，培养的Sertoli细胞暴露于BPA后，细胞活力下降，呈剂量和时间依赖关系，暴露于BPA可引起细胞的形态改变，如膜泡、细胞圆形、细胞骨架塌陷、染色质凝集或碎裂，所有这些都符合细胞凋亡的形态标准。免疫细胞化学结果显示，主要执行半胱氨酸天冬氨酸氨基转移酶的活性caspase-3在TUNEL法阳性标记的圆形支持细胞中表达。在圆形支持细胞中，caspase-3活性片段和聚集的肌动蛋白片段也共存。以上结果提示，双酚A通过促进细胞凋亡而诱导支持细胞死亡。双酚A可诱导细胞死亡，而双酚A在100μ时对细胞仅有轻微的细胞毒作用。(C)2003 Elsevier Science Inc.保留所有权利。
Bisphenol A (BPA) was examined for its effects on cultured Sertoli cells established from 18-day-old rat testes. We demonstrated that exposure of cultured Sertoli cells to BPA decreased the cell viability in a dose- and a time-dependent manner and that exposure to BPA brought about morphologic changes of the cells, such as membrane blebs, cell rounding, cytoskeletal collapse, and chromatin condensation or fragmentation, all of which conform to the morphologic criteria for apoptosis. Immunocytochemistry showed that active caspase-3, a major execution caspase, was expressed in round Sertoli cells positively labeled by the TUNEL method. Co-localization of active caspase-3 and aggregated actin fragments was also observed in the round Sertoli cells. Theses results suggest that BPA induces cell death of Sertoli cells by promoting apoptosis. Apoptosis-inducing cell death was observed in cells exposed to 150-200 muM BPA, while BPA at < 100 μM had only slight cytotoxic effects on the cells. (C) 2003 Elsevier Science Inc. All rights reserved.