Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells.
Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells.
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双色 FISH 分析表征细胞色素 P450 2B1 重组 V79 中国仓鼠细胞中的标记染色体。
DOI：
10.1093/mutage/14.1.57
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发表时间：
1999
期刊：
影响因子：
2.7
通讯作者：
F. Darroudi
中科院分区：
文献类型：
--
作者：
S. Simi;Y. Xiao;M. Campagna;J. Doehmer;F. Darroudi
为研究新陈代谢毒性，构建了表达细胞色素P450等有用功能的重组V79中国仓鼠细胞系。重组细胞系通过基因转移和从亲本V79细胞系中筛选得到单克隆。保持亲本系原有的生物学特性对重组细胞系的可靠应用具有重要意义。作为这些工作的一部分，与中国仓鼠染色体特异性DNA文库进行了荧光原位杂交，以鉴定重组细胞系V79MZr2B1中存在的标记染色体的来源，而标准细胞遗传学技术无法鉴定该染色体的来源。
Recombinant V79 Chinese hamster cell lines have been constructed for the expression of useful functions, e.g. cytochromes P450, in order to study metabolism-dependent toxicity. Recombinant cell lines are derived as single clones upon gene transfer and selection from the parental V79 cell line. It is of fundamental importance for a reliable application of the recombinant cell lines to show that the original biological characteristics of the parental line are maintained. As part of these efforts, fluorescence in situ hybridization with Chinese hamster chromosome-specific DNA libraries was performed in order to identify the origin of the chromosomes from which a marker chromosome present in the recombinant cell line V79MZr2B1 was derived, which could not be identified by standard cytogenetic techniques.