Control of Distal-less expression in the Drosophila appendages by functional 3′ enhancers
Control of Distal-less expression in the Drosophila appendages by functional 3′ enhancers
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DOI：
10.1016/j.ydbio.2011.02.005
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发表时间：
2011-05-15
影响因子：
2.7
通讯作者：
Couso, Juan Pablo
中科院分区：
文献类型：
--
作者：
Galindo, Maximo Ibo;Fernandez-Garza, Diego;Couso, Juan Pablo
Hox基因Distal-less（Dll）的表达指导多种动物中附属物的发育。在果蝇中，它的表达受到复杂的发育控制。在本工作中，我们研究了位于编码序列下游的Dll基因座中的17 kb基因组区域，并发现了对Dll在腿和其他组织中的表达具有主要功能重要性的控制元件。特别感兴趣的是一个控制元件，我们称之为LP，它从早期胚胎发育开始驱动Dll在腿原基中的表达，并且其缺失会导致成人腿的严重截短和畸形。这是第一个Dll增强子，除了驱动报告基因表达的能力之外，还可以证明其在内源Dll基因的表达和腿的发育中的作用。此外，我们的研究结果表明，一些增强子，相反，广泛接受的概念，可能需要一个特定的5'或3'位置相对于转录区。(C)2011年由Elsevier Inc.出版
The expression of the Hox gene Distal-less (Dll) directs the development of appendages in a wide variety of animals. In Drosophila, its expression is subjected to a complex developmental control. In the present work we have studied a 17 kb genomic region in the Dll locus which lies downstream of the coding sequence and found control elements of primary functional importance for the expression of Dll in the leg and in other tissues. Of particular interest is a control element, which we have called LP, which drives expression of Dll in the leg primordium from early embryonic development, and whose deletion causes severe truncation and malformation of the adult leg. This is the first Dll enhancer for which, in addition to the ability to drive expression of a reporter, a role can be demonstrated in the expression of the endogenous Dll gene and in the development of the leg. In addition, our results suggest that some enhancers, contrary to the widely accepted notion, may require a specific 5' or 3' position with respect to the transcribed region. (C) 2011 Published by Elsevier Inc.