Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells
Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells
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DOI：
10.1021/jf0107252
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发表时间：
2001-11-01
影响因子：
6.1
通讯作者：
Wang, SY
中科院分区：
文献类型：
--
作者：
Chen, YJ;Shiao, MS;Wang, SY
咖啡酸苯乙酯(CAPE)是从蜂胶中分离得到的一种有效成分，本研究旨在探讨CAPE诱导人白血病HL-60细胞凋亡的机制。结果发现，CAPE快速进入HL-60细胞，并以浓度和时间依赖的方式抑制其存活。CAPE诱导了这些细胞特有的DNA片段化和典型的细胞凋亡的形态变化。细胞凋亡率在CAPE(6µg/mL)作用后呈时间依赖性增加(72 h达66.7+/-2.0%)。CAPE作用人白血病HL-60细胞4h后，caspase-3迅速激活，6h后Bcl2表达下调，16h后Bax表达上调，提示CAPE是一种有效的细胞凋亡诱导剂，其作用伴随着caspase-3的激活、Bcl2的下调和Bax的上调。
Caffeic acid phenethyl ester (CAPE) is an active component isolated from propolis, The aim of this study was to investigate the mechanism of CAPE-induced apoptosis in human leukemic HL-60 cells. It was found that CAPE entered HL-60 cells very quickly and then inhibited their survival in a concentration- and time-dependent manner. CAPE induced characteristic DNA fragmentation and morphological changes typical of apoptosis in these cells. Estimation of the apoptotic percentage showed a time-dependent increase after CAPE (6 mug/mL) treatment (up to 66.7 +/- 2.0% at 72 h). Treatment with CAPE caused rapid activation of caspase-3 after 4 h, down-regulation of Bcl-2 expression after 6 h, and up-regulation of Bax expression after 16 h. These results suggest that CAPE is a potent apoptosis-inducing agent; its action is accompanied by activation of caspase-3, down-regulation of Bcl-2, and up-regulation of Bax in human leukemic HL-60 cells.