INDUCTION OF CD14 ANTIGEN ON THE SURFACE OF U937 CELLS BY AN INTERLEUKIN-6 AUTOCRINE MECHANISM AFTER CULTURE WITH FORMALIN-KILLED GRAM-NEGATIVE BACTERIA
INDUCTION OF CD14 ANTIGEN ON THE SURFACE OF U937 CELLS BY AN INTERLEUKIN-6 AUTOCRINE MECHANISM AFTER CULTURE WITH FORMALIN-KILLED GRAM-NEGATIVE BACTERIA
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DOI：
10.1111/j.1399-0039.1991.tb02024.x
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发表时间：
1991-09-01
期刊：
影响因子：
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通讯作者：
INOKO, H
中科院分区：
文献类型：
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作者：
IKEWAKI, N;INOKO, H
为了更好地了解单核细胞样细胞系U937表面CD 14抗原对细菌应答的调节，使用单克隆抗体MY-4测定并通过间接免疫荧光法分析了这些细胞在与福尔马林灭活的细菌一起培养时表面CD 14抗原的表达和调节。 在用本研究中使用的所有福尔马林杀死的革兰氏阴性细菌但不用革兰氏阳性细菌培养约48小时后，在U937细胞上诱导CD 14表达。 用福尔马林灭活的沙门氏菌菌株116-54培养后获得最大表达。 使用酶免疫测定或放射免疫测定系统，在用或不用福尔马林杀死的沙门氏菌116-54培养的U937细胞的培养上清液中测定各种细胞因子，例如白细胞介素-1-β、白细胞介素-2、白细胞介素-6、干扰素-γ和肿瘤坏死因子-α。 发现U937细胞产生大量的白细胞介素-6以响应福尔马林杀死的沙门氏菌116-54。 另一方面，从用福尔马林灭活的沙门氏菌116-54培养72小时后的U937细胞获得的培养上清液（称为条件培养基）在48至72小时后也诱导了CD 14抗原的强表达，这通过添加抗人白细胞介素-6抗体而被阻断。 这些发现表明，与福尔马林杀死的革兰氏阴性菌培养的U937细胞表面上的CD 14抗原的表达是由白细胞介素-6诱导的，并且可以根据白细胞介素-6的自分泌机制来解释。
In order to better understand the regulation of CD14 antigen on the surface of the monocyte-like cell line U937 in response to bacteria, the expression and regulation of CD14 antigen on these cells when cultured with formalin-killed bacteria were determined using the monoclonal antibody MY-4 and analyzed by means of the indirect immunofluorescence method. CD14 expression was induced on the U937 cells after about 48 hours of culture with all of the formalin-killed Gram-negative bacteria used in this study but with none of the Gram-positive bacteria. Maximum expression was obtained after culture with formalin-killed Salmonella enteritidis strain 116-54. Various cytokines such as interleukin-1-beta, interleukin-2, interleukin-6, interferon-gamma and tumor-necrosis factor-alpha were assayed in the culture supernatant of U937 cells cultured with or without formalin-killed Salmonella enteritidis 116-54 using an enzyme-immunoassay or radioimmunoassay system. The U937 cells were found to produce a large amount of interleukin-6 in response to formalin-killed Salmonella enteritidis 116-54. On the other hand, culture supernatant (referred to as conditioned medium) obtained from the U937 cells after 72 h of culture with formalin-killed Salmonella enteritidis 116-54 also induced strong expression of CD14 antigen 48 to 72 h later, and this was blocked by the addition of anti-human interleukin-6 antibody. These findings suggest that the expression of CD14 antigen on the surface of U937 cells cultured with formalin-killed Gram-negative bacteria is induced by interleukin-6 and can be explained on the basis of the autocrine mechanism of interleukin-6.