Genetic Variation of 6-Phosphogluconate Dehydrogenase, Isocitrate Dehydrogenase, and Glutamic-axaloacetic Transaminase in the Liver of Japanese Eel*1
Genetic Variation of 6-Phosphogluconate Dehydrogenase, Isocitrate Dehydrogenase, and Glutamic-axaloacetic Transaminase in the Liver of Japanese Eel*1
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日本鳗鱼肝脏中6-磷酸葡萄糖酸脱氢酶、异柠檬酸脱氢酶和谷氨酸-轴酰乙酸转氨酶的遗传变异*1
DOI：
10.2331/suisan.44.1351
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
K. Numachi
中科院分区：
文献类型：
--
作者：
N. Taniguchi;K. Numachi
对产自中国富知县和高知县的两组日本鳗的肝脏酶谱变化进行了分析。6-磷酸葡萄糖脱氢酶(6-PGD)、异柠檬酸脱氢酶(ICD)和谷草转氨酶(GOT)基因变异明显。在编码6-PGD酶的一个基因座上假设有5个等位基因，从而解释了6-PGD中检测到的10种不同的表型。这五个等位基因中的两个在两个样本中都被检测到相对较高的频率。推测ICD的8种表型是由一个基因座上的4个等位基因引起的。Icde等位基因在两个样本中最常见。这三个或四个不同的GOT的大多数阴极系统表现出三种表型，推测这三种表型是由GOT-III基因座上的两个等位基因控制的。除低频率(0.073和0.120)的Lcdb外，两组间的等位基因频率差异无统计学意义。
Two groups of the Japanese eel, Anguilla japonica, one from Fuchien, China, and the other from Kochi, were analyzed for electrophoretic variation of enzymes in liver. Distinct geneti c variations were found in 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (ICD), and glutamic-oxaloacetic transaminase (GOT). Ten different phenotypes detected in 6- PGD were accounted for by assuming five alleles at a locus encoding this enzyme. Two of the five alleles were detected in relatively high frequencies in both the samples. Eight phenotypes of ICD were postulated to result from four alleles at a locus. Icde allele was most common in the two samples. The most cathodal system of the three or four different ones of GOT exhibited three phenotypes, which were presumed to be controlled by two alleles at a locus termed Got-III. Stati stically significant differences in the allele frequencies could not be observed between the two samples except those of lcdb, which were detected in low frequencies (0.073 and O.120) in the samples.