K-ras mutation in colorectal cancer: relations to patient age, sex and tumour location.
K-ras mutation in colorectal cancer: relations to patient age, sex and tumour location.
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DOI：
10.1038/bjc.1994.67
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发表时间：
1994-02
影响因子：
8.8
通讯作者：
Gaudernack, G
中科院分区：
文献类型：
--
作者：
Breivik, J;Meling, G I;Spurkland, A;Rognum, T O;Gaudernack, G
从123名男性和125名女性挪威结直肠癌患者中获得的251个原发性肿瘤的DNA进行了分析，以确定密码子12和13处是否存在K-ras点突变。在99个（39%）样本中发现了突变。K-ras基因突变的频率与患者的年龄和性别以及肿瘤的位置显著相关（总体：P = 0.008）。在年轻的结肠肿瘤患者中，男性患者的K-ras突变频率远低于女性患者（< 40岁，比值比< 0.014）。低频率可能表明，一个不同的，ras独立的，肿瘤形成的途径是占主导地位的年轻男性的结肠。相反，老年男性比老年女性有更多的突变（例如90岁，比值比= 5.8）。相反，但不太明显的关系被视为直肠肿瘤。发现突变类型与患者的性别和肿瘤的位置有关。G->C颠换占女性直肠肿瘤突变的35%，而在其他材料中仅占2.5%（P = 0.0005）。这可能表明有特定的致癌物质在这个位置起作用。
DNA from 251 primary tumours obtained from 123 male and 125 female Norwegian patients with colorectal carcinoma was analysed for the presence of K-ras point mutations at codons 12 and 13. Mutations were found in 99 (39%) of the samples. The frequency of K-ras mutations was significantly related to age and sex of the patients, and to the location of the tumours (overall: P = 0.008). K-ras mutations were much less frequent in colonic tumours from male than female patients at younger ages (< 40 years, odds ratio < 0.014). The low frequency might indicate that a different, ras-independent, pathway to neoplasia is dominating in the colon of younger males. In contrast, older men had more mutations than older women (e.g. 90 years, odds ratio = 5.8). An inverse but less pronounced relationship was seen for rectal tumours. The type of mutation was found to be associated to sex of patient and location of tumour. G-->C transversions accounted for 35% of the mutations in rectal tumours from females, in contrast to only 2.5% in the rest of the material (P = 0.0005). This may indicate that there are specific carcinogens acting in this location.