"Shutter" Effects Enhance Protein Diffusion in Dynamic and Rigid Molecular Networks.
"Shutter" Effects Enhance Protein Diffusion in Dynamic and Rigid Molecular Networks.
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DOI：
10.1021/jacs.2c07830
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发表时间：
2022-10
影响因子：
15
通讯作者：
X. Dai;Zhichao Zhu;Yujie Li;Bo Yang;Jiangfei Xu;Yuanchen Dong;Xin Zhou;Li-Tang Yan;Dongsheng Liu
中科院分区：
文献类型：
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作者：
X. Dai;Zhichao Zhu;Yujie Li;Bo Yang;Jiangfei Xu;Yuanchen Dong;Xin Zhou;Li-Tang Yan;Dongsheng Liu
水凝胶被广泛应用于了解天然细胞外基质（ECM）的基本功能和机制。然而，通过合成水凝胶来揭示ECM的高渗透性仍然面临着构建具有刚性和动态性质的模拟网络的挑战。在本研究中，利用DNA构建块的刚性和动态结合，我们设计了一种与ECM结构相似的模型水凝胶系统，与合成聚丙烯酰胺（PAAm）水凝胶相比，它可以增强蛋白质的扩散。通过实验和模拟研究了分子在这种刚性动态网络中的扩散行为，扩散系数与分子大小的关系表现出从幂律到指数函数的独特转变。提出了一种基于刚性和动态分子网络的“快门”模型，该模型成功揭示了刚性和动态键交换如何决定扩散机制，可能为理解ECM中增强扩散行为的可能机制提供了新的视角。
Hydrogels have been widely applied to understand the fundamental functions and mechanism of a natural extracellular matrix (ECM). However, revealing the high permeability of ECM through synthetic hydrogels is still challenged by constructing analogue networks with rigid and dynamic properties. Here, in this study, taking advantage of the rigidity and dynamic binding of DNA building blocks, we have designed a model hydrogel system with structural similarity to ECM, leading to enhanced diffusion for proteins compared with a synthetic polyacrylamide (PAAm) hydrogel. The molecular diffusion behaviors in such a rigid and dynamic network have been investigated both in experiments and simulations, and the dependence of diffusion coefficients with respect to molecular size exhibits a unique transition from a power law to an exponential function. A "shutter" model based on the rigid and dynamic molecular network has been proposed, which has successfully revealed how the rigidity and dynamic bond exchange determine the diffusion mechanism, potentially providing a novel perspective to understand the possible mechanism of enhanced diffusion behaviors in ECM.