Genetic diversity in the Churra tensina and Churra lebrijana endangered Spanish sheep breeds and relationship with other Churra group breeds and Spanish mouflon
Genetic diversity in the Churra tensina and Churra lebrijana endangered Spanish sheep breeds and relationship with other Churra group breeds and Spanish mouflon
复制标题
DOI：
10.1016/j.smallrumres.2010.09.003
复制
发表时间：
2011-01-01
影响因子：
1.8
通讯作者：
Sanz, A.
中科院分区：
文献类型：
--
作者：
Calvo, J. H.;Alvarez-Rodriguez, J.;Sanz, A.
本研究利用28个微卫星标记，分析了中国甜筒菊（Churra tensina）和印度甜筒菊（Churra lebrijana）濒危品种的遗传变异，并与中国甜筒菊、拉丁美洲甜筒菊、美利奴品种和西班牙甜筒菊建立了亲缘关系，为中国甜筒菊和印度甜筒菊的保护提供了有用的信息。等位基因频率和杂合性揭示了高的遗传变异，在两个濒危品种，尽管他们的人口规模小。估计的近交系数（F（IS））是显着的所有品种的研究，除了Churra lebrijana品种。最高的近交系数（F（IS）=0.143）被发现在西班牙的mouches。遗传分化测试（F（ST）=0.121）和分配的个人的人口表明存在定义的品种种群，这些品种之间的低遗传流。最高的成对雷诺距离（D（R））值之间观察到的Moulanus和国内绵羊品种。仅考虑国内绵羊品种，Churra lebrijana品种表现出最高的成对D（R）值。最低值之间发现Latxa和其他国内绵羊，除了Churra lebrijana。成对D（R）值的结果，以及系统发育树和瓶颈分析表明，一个重要的遗传隔离的Churra lebrijana品种从其他Churra类型，和遗传特征的人口瓶颈。最后，群体结构分析发现在Latxa绵羊品种的群体细分。总之，本研究提出了有价值的洞察到现有的遗传变异的两个西班牙濒危品种，以及第一次研究在西班牙的moutheles基于微卫星分析。在Churra tensina和Churra lebrijana中表现出的高度变异性意味着这些种群是遗传多样性的丰富储存库。(C)2010 Elsevier BV保留所有权利。
The aim of the present study was to estimate the genetic intra-breed variability of Churra tensina and Churra lebrijana endangered breeds and to establish genetic relationships with Churra, Latxa and Merino breeds, as well as Spanish mouflon, by using 28 microsatellite markers, to provide useful information for their conservation. Allele frequencies and heterozygosity revealed high genetic variation in the two endangered breeds despite their small population size. Estimates of inbreeding coefficient (F(IS)) were significant for all breeds studied, except for Churra lebrijana breed. The highest inbreeding coefficient (F(IS)=0.143) was found in the Spanish mouflon. Genetic differentiation tests (F(ST)=0.121) and assignment of individuals to populations indicated the existence of defined breed populations, and low genetic flow between these breeds. The highest pairwise Reynolds distance (D(R)) values were observed between Mouflon and the domestic sheep breeds. Considering only domestic sheep breeds, the Churra lebrijana breed showed the highest pairwise D(R) values. The lowest values were found between Latxa and the other domestic sheep, except for Churra lebrijana. Results of pairwise D(R) values, as well as phylogenetic tree and bottleneck analysis showed an important genetic isolation of the Churra lebrijana breed from the other Churra types, and genetic signatures of a demographic bottleneck. Finally, structure analysis of populations detected a population subdivision in the Latxa sheep breed. In conclusion, this study presents valuable insight into the existing genetic variability of two Spanish endangered breeds, as well as the first study in Spanish mouflon based on microsatellite analysis. The high degree of variability demonstrated in Churra tensina and Churra lebrijana implies that these populations are rich reservoirs of genetic diversity. (C) 2010 Elsevier B.V. All rights reserved.