BRB, A PLATELET ALLOANTIGEN INVOLVED IN NEONATAL ALLOIMMUNE THROMBOCYTOPENIA
BRB, A PLATELET ALLOANTIGEN INVOLVED IN NEONATAL ALLOIMMUNE THROMBOCYTOPENIA
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DOI：
10.1111/j.1423-0410.1991.tb00911.x
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发表时间：
1991-01-01
期刊：
影响因子：
2.7
通讯作者：
BIERLING, P
中科院分区：
文献类型：
--
作者：
BETTAIEB, A;FROMONT, P;BIERLING, P
一名患有May-Hegglin异常的孕妇的血清中含有血小板特异性抗体。在血小板间接免疫荧光试验（PIIFT）中，血清与97.6%的随机供者血小板和父亲的血小板反应，而与母亲的血小板不反应。该抗体诱导婴儿出现中度血小板减少症，对静脉输注浓缩免疫球蛋白有反应。母亲血小板表型为PLA 1+，巴克（a）+，Br（a）+/Br（b）-，父亲为PLA 1+，巴克（a）+，Br（a）-/Br（B）+，新生儿为PLA 1+，Br（a）+/Br（B）+。基于单克隆抗体固定化血小板抗原（MAIPA）和免疫沉淀技术的免疫测定的母体血清分析表明，不存在针对HLA I类抗原的抗体，并且识别的抗原位于血小板-GpIa/IIa复合物上。该抗原存在于113/115个随机供体血小板上，在7个无关的May-Hegglin血小板中的7个中，并且仅在3/24个Br（a）+个体（包括母亲）中不存在。7名无关的May-Hegglin受试者的血清中不存在血小板特异性抗体。因此，被该血小板特异性抗体识别的抗原符合定义Br（a）的对偶等位基因，即Br（B）同种异体抗原的标准。
Serum from a pregnant woman with the May-Hegglin anomaly contained a platelet-specific antibody. The serum reacted in the platelet indirect immunofluorescence test (PIIFT) with 97.6% of random donor platelets and those of the father but not with the mother's own platelets. This antibody induced a moderate thrombocytopenia in the infant that responded to infusion of intravenous immunoglobulin concentrates. The platelet phenotypes were PLA1+, Bak(a)+, Br(a)+/Br(b)- for the mother, PLA1+, Bak(a)+, Br(a)-/Br(b)+ for the father, and PLA1+, Br(a)+/Br(b)+ for the neonate. Analysis of the maternal serum with an immunoassay based on monoclonal antibody immobilization of platelet antigens (MAIPA) and immunoprecipitation techniques demonstrated the absence of antibodies directed against HLA class I antigens and that the antigen recognized was located on the platelet-GpIa/IIa complex. This antigen was present on 113/115 random donor platelets, in 7 of the 7 unrelated May-Hegglin platelets, and only absent in 3/24 Br(a)+ individuals, including the mother. No platelet-specific antibody was present in the serum of the 7 unrelated May-Hegglin subjects. The antigen recognized by this platelet-specific antibody thus meets the criteria defining the antithetic allele of Br(a), i.e. the Br(b) alloantigen.