Interaction of lactic acid bacteria with the gut immune system
Interaction of lactic acid bacteria with the gut immune system
复制标题
DOI：
10.1038/sj.ejcn.1601658
复制
发表时间：
2002-12-01
影响因子：
4.7
通讯作者：
Medici, M
中科院分区：
文献类型：
--
作者：
Perdigón, G;Galdeano, CM;Medici, M
用于功能性食品和药物制剂的乳酸菌（LAB）的健康声明是基于这些微生物刺激宿主免疫系统的能力。本研究以嗜酸乳杆菌（Lactobacillus acidophilus）、干酪乳杆菌（Lactobacillus casei）、德氏乳杆菌（Lactobacillus delbrueckii ssp.保加利亚链球菌和嗜热链球菌）对BALB/c小鼠肠道免疫系统的影响。所有LAB检测均观察到Bcl 2蛋白呈剂量依赖性增加。此外，对肠道固有层中的精氨酸产生细胞的分析表明，在从派尔集合淋巴结培养的巨噬细胞中测定的TNF α和INF γ值对于所有测定的LAB均增强。白细胞介素IL-10和IL-4的重要增加主要在喂食德氏乳杆菌亚种的小鼠中观察到。保加利亚乳杆菌或干酪乳杆菌对IL-2和IL-12有显著的诱导作用。嗜酸乳杆菌（P < 0.01）。这些效应具有剂量依赖性。产生的细胞因子在平衡Th 1/Th 2中的作用由针对肠胃外注射的卵清蛋白的全身性抗体应答确定。L.干酪湖（L. casei）德氏bulgaricus和L.嗜酸乳杆菌增强IgG 1应答，有利于Th 2平衡，而L.嗜酸乳杆菌还增加IgG 2a应答，诱导Th 1平衡。S.嗜热菌对Th 1/Th 2平衡无影响。我们的研究表明，乳酸菌诱导不同的粘膜细胞因子谱，显示它们之间不同的佐剂能力。因此，具有免疫学特性的益生菌菌株的选择必须被很好地定义以影响有利于所要求的免疫应答的细胞因子表达。
Health claims of lactic acid bacteria (LAB) used in functional foods and pharmaceutical preparations are based on the capacity of these microorganisms to stimulate the host immune system. In this study, the antigenic effect of LAB (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) on the gut immune system of BALB/c mice was evaluated. A dose-dependent increase of the Bcl2 protein was observed with all LAB assayed. Furthermore, the analysis of cytokine-producing cells in the lamina propria of gut showed that TNFalpha and INFgamma values, determined in macrophages cultured from Peyer patches, were enhanced for all the LAB assayed. An important increase of interleukins IL-10 and IL-4 was observed mainly in mice fed with Lactobacillus delbrueckii ssp. bulgaricus or Lactobacillus casei, while a significant induction of IL-2 and IL-12 was only observed with L. acidophilus (P < 0.01). These effects were dose dependent. The role of produced cytokines in the balance Th1/Th2 was determined by a systemic antibody response against parenterally injected ovoalbumin. L. casei, L. delbrueckii ssp. bulgaricus and L. acidophilus enhanced the lgG1 response favouring Th2 balance, while L. acidophilus also increased the IgG2a response inducing Th1 balance. S. thermophilus did not influence the balance Th1/Th2. Our studies showed that lactic acid bacteria induce distinct mucosal cytokine profiles showing different adjuvant capacity among them. Thus, selection of probiotic strain with immunological properties must be well defined to influence cytokine expression that favour the claimed immune response.