The effect of licochalcone A on cell-aggregates ECM secretion and osteogenic differentiation during bone formation in metaphyseal defects in ovariectomized rats
The effect of licochalcone A on cell-aggregates ECM secretion and osteogenic differentiation during bone formation in metaphyseal defects in ovariectomized rats
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甘草查耳酮 A 对卵巢切除大鼠干骺端缺损骨形成过程中细胞聚集 ECM 分泌和成骨分化的影响。
DOI：
10.1016/j.biomaterials.2013.12.061
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发表时间：
2014-03-01
期刊：
影响因子：
14
通讯作者：
Jin, Yan
中科院分区：
文献类型：
--
作者：
Shang, Fenqing;Ming, Leiguo;Jin, Yan
负重性骨折伴骨缺损的治疗是骨质疏松症患者康复的关键。虽然各种组织工程方法被报道，但组织工程的最佳治疗策略仍有待确定，因为提高种子细胞的成骨分化潜能的能力的局限性是需要解决的主要问题之一。本研究旨在探讨甘草查尔酮-A（L-A）和骨髓间充质干细胞（BMSC）-聚集体在骨修复组织工程中应用的可行性，并进一步研究甘草查尔酮-A对细胞聚集体形成和成骨特性的生物学效应。雌性SD道利大鼠80只，切除双侧卵巢，造成股骨3.5mm骨缺损，不加任何固定。然后将这些大鼠随机分为5个不同的治疗组：（1）空缺损组（n = 16），（2）CA-LA组（n = 16），（3）CA-N组（n = 16），（4）CA-L组（n = 16），（5）CA-S组（n = 16），16只雌性SD大鼠作为对照组。结果表明，L-A处理组的成骨分化能力和矿化能力均强于未处理组。在体外实验中，与对照组相比，L-A组有更高的ECM、成骨相关标志物的表达，矿化面积更大，ALP活性更高。在体研究方面，显微CT三维重建、HE染色和骨强度结果显示，L-A组移植后2、4、8、12周的新骨形成明显高于Sham组，尤其是8周时全身注射L-A组。我们的研究结果表明，LA可以积极影响细胞聚集体工程中的细胞行为，并可能是一个有前途的策略，在治疗骨质疏松性承重骨骨折缺损。(C)2014爱思唯尔有限公司版权所有。
Treatment of weight-bearing bones fractures with defects is critical for patients with osteoporosis's rehabilitation. Although various tissue engineering methods were reported, the best treating strategy for tissue engineering remains to be identified as the limitation of enhancing the ability of the osteogenetic differentiation potential of seed cell is one of the cardinal issues to be solved. The objective of this study is to investigate the feasibility of applying licochalcone-A (L-A) and bone marrow mesenchymal stem cells (BMSC)-aggregate in bone repairing tissue engineering and further study the biological effects of LA on the cell aggregate formation and osteogenic properties. 80 female Sprague Dawley rats underwent bilateral ovariectomy were made with a 3.5 mm femurs bone defects without any fixation. These rats were then randomly assigned to five different treatment groups: (1) empty defect (n = 16), (2) CA-LA (n = 16), (3) CA-N (n = 16), (4) CA-L (n = 16), (5) CA-S (n = 16) and 16 female SD rats were treated as a control. Data showed that L-A administrated cell aggregate had a stronger osteogenic differentiation and mineralized formation potential than non-administrated group both in vitro and in vivo. For in vitro study, L-A administrated group had a more significant expression of ECM, osteogenic associated maker in addition with more mineralized area and higher ALP activity compared with the control group. For in vivo study, 3D reconstruction of micro-CT, HE staining and bone strength results showed that newly formed bone in groups administrated by L-A was significant higher than that in Sham group at 2, 4, 8 and 12 weeks after transplantation, especially for groups which was systematically injected with L-A at 8 weeks. Results of our study demonstrated that LA could positively affect cell behavior in cell-aggregate engineering and could be a promising strategy in treating osteoporotic weight-bearing bones fractures with defects. (C) 2014 Elsevier Ltd. All rights reserved.