Determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in human serum using programmable-temperature vaporization gas chromatography with high-resolution mass spectrometry.
Determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in human serum using programmable-temperature vaporization gas chromatography with high-resolution mass spectrometry.
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DOI：
10.1002/jssc.201700465
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发表时间：
2017-09
影响因子：
3.1
通讯作者：
Lei Zhang;Yuxin Zhong;Xin Liu;Yan Bao;Yun-feng Zhao;Yongning Wu;Z. Cai;Jingguang Li
中科院分区：
文献类型：
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作者：
Lei Zhang;Yuxin Zhong;Xin Liu;Yan Bao;Yun-feng Zhao;Yongning Wu;Z. Cai;Jingguang Li
测定非职业人群血液中的多氯二苯并对二恶英和多氯二苯并呋喃以及二恶英样多氯联苯对于调查这些污染物对健康的不良影响至关重要。在这项研究中，一个灵敏的方法的基础上，程序升温蒸发与大体积注射耦合气相色谱-高分辨率质谱法测定这些污染物在1-2 mL的人血清样品。采用析因设计对程序升温汽化注射器的排气温度、排气时间、排气流量、转移温度和转移时间等关键参数进行了优化。通过测定校准标准溶液、标准参考物质和非职业人群真实的人体血清样品中的多氯二苯并对二恶英、多氯二苯并呋喃和二恶英样多氯联苯，评价了方法的准确度、精密度和适用性。多氯二苯并二恶英、多氯二苯并呋喃和二恶英类多氯联苯的方法检出限分别为1.5-9.0和0.005-0.02 ng/kg湿重。与常规不分流进样相比，程序升温汽化进样器的应用可有效提高1-2 mL人血清样品中二恶英类多氯联苯、二恶英类多氯联苯和多氯二苯并呋喃的检出率。
The determination of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in blood from a non-occupational population is essential for the investigation of adverse health effects from these pollutants. In this study, a sensitive method based on programmable-temperature vaporization with large-volume injection coupled with gas chromatography with high-resolution mass spectrometry was developed to determine these pollutants in 1-2 mL of human serum samples. Various key parameters of programmable-temperature vaporization injector, including vent temperature, vent time, vent flow, transfer temperature and transfer time were optimized by factorial design. The accuracy and precision as well as applicability were assessed by determining polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in calibration standard solutions, standard reference materials and real human serum samples from non-occupational population. The method detection limits of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls were 1.5-9.0 and 0.005-0.02 ng/kg wet weight, respectively. By comparing with typically splitless injection, the application of programmable-temperature vaporization injector could effectively lead to higher detectable rate of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in 1-2 mL of human serum samples.