Reduction and uptake of methylene blue by human erythrocytes
Reduction and uptake of methylene blue by human erythrocytes
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DOI：
10.1152/ajpcell.00512.2003
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发表时间：
2004-06-01
影响因子：
5.5
通讯作者：
Cobb, CE
中科院分区：
文献类型：
--
作者：
May, JM;Qu, ZC;Cobb, CE
已经在内皮细胞中描述了一种噻吩染料还原酶，它可以减少亚甲基蓝（MB），使其进入细胞。由于提出了人红细胞中MB摄取的不同机制，我们测量了这种细胞类型的MB摄取和减少。氧化的MB （MB+）以时间和浓度依赖的方式刺激细胞外铁氰化物的还原，反映了染料的细胞外还原。还原后的MB被细胞吸收，部分氧化为MB+。这两种形式在浓度梯度下都保持不变，它们的氧化还原循环在细胞中诱导氧化应激。而MB+的浓度
A thiazine dye reductase has been described in endothelial cells that reduces methylene blue (MB), allowing its uptake into cells. Because a different mechanism of MB uptake in human erythrocytes has been proposed, we measured MB uptake and reduction in this cell type. Oxidized MB (MB+) stimulated reduction of extracellular ferricyanide in a time- and concentration-dependent manner, reflecting extracellular reduction of the dye. Reduced MB was then taken up by the cells and partially oxidized to MB+. Both forms were retained against a concentration gradient, and their redox cycling induced an oxidant stress in the cells. Whereas concentrations of MB+