Functional recovery after human umbilical cord blood cells transplantation with brain-derived neutrophic factor into the spinal cord injured rat
Functional recovery after human umbilical cord blood cells transplantation with brain-derived neutrophic factor into the spinal cord injured rat
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DOI：
10.1007/s00701-005-0538-y
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发表时间：
2005-09-01
影响因子：
2.4
通讯作者：
Ha, Y
中科院分区：
文献类型：
--
作者：
Kuh, SU;Cho, YE;Ha, Y
在脊髓损伤后恢复神经功能方面已经有很多努力，但在脊髓损伤的治疗方面存在一定的局限性。神经干细胞以其多能性分化为各种神经细胞类型而著称。人类脐带血细胞（HUCBs）比骨髓神经干细胞更具多能性和遗传灵活性。hucb可以在未来更频繁地用于脊髓损伤治疗。将中度脊髓损伤大鼠分为3个亚组，A组：在脊髓损伤部位注射培养基，B组：将HUCBs移植到脊髓损伤部位，C组：将含脑源性中性营养因子的HUCBs移植到脊髓损伤部位。我们在移植后8周检查各组BBB评分以评估功能恢复情况。最后用双免疫荧光染色检测神经细胞分化，用荧光金逆行标记脑干神经元分析轴突再生。hucb移植组在移植后每周均较对照组改善，且BDNF自应用后1周起使BBB运动评分改善（P < 0.05）。移植后8周，hubs合并BDNF移植组BBB评分较其他组明显改善（P < 0.001）。移植的hubs分化为多种神经细胞，经BrdU双免疫荧光染色和GFAP & MAP-2染色证实。hucb和BDNF对轴突再生都有各自的积极作用。在脊髓损伤大鼠模型中，hubs可分化为神经细胞并诱导运动功能改善。特别是，BDNF由于轴突再生在早期脊髓损伤阶段具有改善神经功能的有效性。因此，HUCBs和BDNF对脊髓损伤大鼠具有中等程度的恢复作用。
There have been many efforts to recover neuronal function from spinal cord injuries, but there are some limitations in the treatment of spinal cord injuries.The neural stem cell has been noted for its pluripotency to differentiate into various neural cell types. The human umbilical cord blood cells (HUCBs) are more pluripotent and genetically flexible than bone marrow neural stem cells. The HUCBs could be more frequently used for spinal cord injury treatment in the future.Moderate degree spinal cord injured rats were classified into 3 subgroups, group A: media was injected into the cord injury site, group B: HUCBs were transplanted into the cord injury site, and group C: HUCBs with BDNF (Brain-derived neutrophic factor) were transplanted into the cord injury site. We checked the BBB scores to evaluate the functional recovery in each group at 8 weeks after transplantation. We then, finally checked the neural cell differentiation with double immunofluorescence staining, and we also analyzed the axonal regeneration with retrograde labelling of brain stem neurons by using fluorogold. The HUCBs transplanted group improved, more than the control group at every week after transplantation, and also, the BDNF enabled an improvement of the BBB locomotion scores since the 1 week after its application (P < 0.05). 8 weeks after transplantation, the HUCBs with BDNF transplanted group had more greatly improved BBB scores, than the other groups (P < 0.001). The transplanted HUCBs were differentiated into various neural cells, which were confirmed by double immunoflorescence staining of BrdU and GFAP & MAP-2 staining. The HUCBs and BDNF each have individual positive effects on axonal regeneration. The HUCBs can differentiate into neural cells and induce motor function improvement in the cord injured rat models. Especially, the BDNF has effectiveness for neurological function improvement due to axonal regeneration in the early cord injury stage. Thus the HUCBs and BDNF have recovery effects of a moderate degree for cord injured rats.